Polyhydroxyalkanoate production potential of heterotrophic bacteria in activated sludge
Polyhydroxyalkanoate production potential of heterotrophic bacteria in activated sludge
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DOI：
10.1016/j.jbiosc.2015.04.022
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发表时间：
2016-01-01
影响因子：
2.8
通讯作者：
Sei, Kazunari
中科院分区：
文献类型：
--
作者：
Inoue, Daisuke;Suzuki, Yuta;Sei, Kazunari
通过对活性污泥中可培养异养菌的基因型和表型特征进行分析，研究其生产聚羟基脂肪酸酯（PHA）的潜力。从实验室规模的序批式反应器和两个城市污水处理厂的三个污水处理工艺的四个活性污泥样品中分离出共114株细菌菌株。PCR检测的phaC基因编码的I类和II类PHA合酶显示，15%的总分离物具有phaC基因，所有这些都具有最接近的相似性，已知的phaC基因的α-和β-变形菌和放线菌。在有氧和氮限制条件下的PHA生产实验表明，68%的总分离物能够从所使用的六种底物（乙酸盐，丙酸盐，乳酸盐，丁酸盐，葡萄糖和甘油）中的至少一种产生PHA。基因型和表型特征表明，75%的活性污泥细菌具有生产PHA的潜力。我们的研究结果还表明，短链脂肪酸将是活性污泥细菌生产PHA的首选底物，并且活性污泥中可能存在多种未知的phaC基因。2015年，日本生物技术学会。All rights reserved.
This study was conducted to evaluate the polyhydroxyalkanoate (PHA) production potential of cultivable heterotrophic bacteria in activated sludge by genotypic and phenotypic characterizations. A total of 114 bacterial strains were isolated from four activated sludge samples taken from a lab-scale sequencing batch reactor and three wastewater treatment processes of two municipal wastewater treatment plants. PCR detection of the phaC genes encoding class I and II PHA synthase revealed that 15% of the total isolates possessed phaC genes, all of which had the closest similarities to known phaC genes of alpha- and beta-Proteobacteria and Actinobacteria. PHA production experiments under aerobic and nitrogen-limited conditions showed that 68% of the total isolates were capable of producing PHA from at least one of the six substrates used (acetate, propionate, lactate, butyrate, glucose and glycerol). Genotypic and phenotypic characterizations revealed that 75% of the activated sludge bacteria had PHA production potential. Our results also indicated that short-chain fatty acids would be the preferable substrates for PHA production by activated sludge bacteria, and that there might be a variety of unidentified phaC genes in activated sludge. 2015, The Society for Biotechnology, Japan. All rights reserved.