Rapid detection and identification of bacteria directly from whole blood with light scattering spectroscopy based biosensor
Rapid detection and identification of bacteria directly from whole blood with light scattering spectroscopy based biosensor
复制标题
利用基于光散射光谱的生物传感器直接从全血中快速检测和鉴定细菌
DOI：
10.1016/j.snb.2021.130489
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Itzkan, Irving
中科院分区：
文献类型：
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作者：
Qiu, Le;Zhang, Lei;Horowitz, Gary L.;Turzhitsky, Vladimir;Coughlan, Mark F.;Glyavina, Maria;Khan, Umar;Zakharov, Yuri N.;Vitkin, Edward;Itzkan, Irving
细菌感染是全世界死亡的主要原因之一。鉴定作为感染源的细菌种类通常需要很长时间，并且通常超过重病患者的治疗窗口。如果能够迅速确定细菌种类，许多死亡是可以预防的。在这里，我们提出了一种使用光散射光谱技术直接从全血中快速检测和鉴定细菌的光学光谱方法。该技术最初被开发用于检测上皮组织中的癌前变化，表征细胞尺度上的组织变化，并表征与单个波长相当或小于单个波长的生物结构。我们在这里证明，不仅可以一个廉价的光散射光谱为基础的生物传感器快速检测和识别血液中的四种细菌物种，负责大多数导致死亡的感染，但物种水平的识别可能是基于每毫升血液约一千个细菌细胞。因此，不需要观察整个菌落或进行敏感性试验。
Bacterial infections are one of the major causes of death worldwide. The identification of a bacterial species that is the source of an infection generally takes a long time, and often exceeds the treatment window for seriously ill patients. Many of these deaths are preventable if the bacterial species can be identified quickly. Here we present an optical spectroscopic method for rapid detection and identification of bacteria directly from whole blood using a light scattering spectroscopy technique. This technique was originally developed to detect pre-cancerous changes in epithelial tissues, characterize changes in tissue on the cellular scale, and characterize biological structures comparable to or smaller than a single wavelength. We demonstrate here that not only can an inexpensive light scattering spectroscopy-based biosensor rapidly detect and identify four bacteria species in the blood, responsible for the majority of death causing infections, but that species-level identification can potentially be made based on approximately one thousand bacterial cells per milliliter of blood. Observing entire colonies or performing susceptibility testing is therefore not required.