Morphological, biological, and molecular characteristics of the diatom Pseudo-nitzschia delicatissima from the Canadian MaritimesThis paper is one of a selection of papers published in the Special Issue on Systematics Research.
Morphological, biological, and molecular characteristics of the diatom Pseudo-nitzschia delicatissima from the Canadian MaritimesThis paper is one of a selection of papers published in the Special Issue on Systematics Research.
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加拿大滨海省硅藻 Pseudo-nitzschia delicatissima 的形态、生物学和分子特征本文是《系统学研究特刊》上发表的论文选集之一。
DOI：
10.1139/b08-046
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发表时间：
2008
期刊：
影响因子：
1.1
通讯作者：
Mónica B. J. Moniz
中科院分区：
文献类型：
--
作者：
I. Kaczmarska;C. Reid;Jennifer L. Martin;Mónica B. J. Moniz
从加拿大东部的沿海沃茨分离了46个硅藻Pseudo-nitzschia delicatissima（Cleve）海登的单克隆培养物。其中，12个克隆人成功实现了性化。它们的形态和遗传差异的范围被用作性别身份仍然未知的克隆的参考。所有的字符进行了检查，包括阀形态，核内转录间隔区（ITS）的区域，和线粒体细胞色素c氧化酶（COX 1）序列，表现出高度的相似性内和交配和无性系之间。在ITS区的638 bp序列中，仅发现5个变异位点，在cox 1基因5′端的576 bp序列中仅发现2个变异位点。我们自己的数据和从GenBank检索到的数据表明，北大西洋北方由遗传上非常相似的P. delicatissima的单一集合种群构成，这是使用该物种表型的ITS序列确定的。ITS…
Forty-six monoclonal cultures of the diatom Pseudo-nitzschia delicatissima (Cleve) Heiden were isolated from coastal waters of Eastern Canada. Of these, 12 clones were successfully sexualized. The range of their morphological and genetic divergence was used as a reference for clones whose sexual identity remains unknown. All characters that were examined, including valve morphology, the nuclear internal transcribed spacer (ITS) region, and mitochondrial cytochrome c oxidase (cox1) sequences, showed a high degree of similarity within and between mating and nonsexualized clones. Within the 638 bp long aligned fragment in the ITS region, only five variable sites were found and just two were found within the 576 bp fragment of cox1 near the 5′ terminus of the gene. Our own data and those retrieved from GenBank suggest that the northern North Atlantic is populated by a single metapopulation of genetically very similar P. delicatissima, as determined using the ITS sequence of the epitype of the species. The ITS...