Powerdress as the novel regulator enhances Arabidopsis seeds germination tolerance to high temperature stress by histone modification of SOM locus.
Powerdress as the novel regulator enhances Arabidopsis seeds germination tolerance to high temperature stress by histone modification of SOM locus.
复制标题
DOI：
10.1016/j.plantsci.2019.04.001
复制
发表时间：
2019-07
期刊：
影响因子：
--
通讯作者：
Wenjuan Yang;Zhen Chen;Yawen Huang;Guanxiao Chang;Ping Li;Jiali Wei;Xiaojun Yuan;Jingling Huang;Xiangyang Hu
中科院分区：
文献类型：
--
作者：
Wenjuan Yang;Zhen Chen;Yawen Huang;Guanxiao Chang;Ping Li;Jiali Wei;Xiaojun Yuan;Jingling Huang;Xiangyang Hu
种子的萌发或休眠受内源激素信号和环境信号的严格控制。高温（HT）抑制种子萌发或引发种子休眠，但其介导种子萌发热抑制的机制还有待进一步研究，SOM是光辐射种子萌发的关键因子，它通过改变阿坝和GA的生物合成来负调控种子的萌发。我们发现HT通过阿坝信号转导元件ABI3促进SOM的表达，ABI3和Som突变体种子在HT处理下的萌发率均高于野生型种子。使用ABI3作为诱饵，我们确定了表观遗传因子Powerdress（PWR）作为ABI3相互作用蛋白。遗传和生理分析表明，PWR负调控SOM的表达，过量表达PWR增强了种子萌发耐热性，而pwr突变体降低了种子萌发耐热性。在无高温胁迫条件下，PWR促进了组蛋白H3去乙酰化水平和H2A.Z在SOM位点的沉积，从而抑制了ABI 3依赖的SOM转录，而高温胁迫则阻断了PWR转录水平，从而减弱了PWR对SOM表达的抑制作用，导致了种子萌发热抑制。因此，我们的发现提出了PWR通过组蛋白乙酰化修饰和H2A. Z沉积控制高温下种子萌发的新功能。
Seeds germination or dormancy is strictly controlled by endogenous phytohormone signal and environment cues. High temperature (HT) suppresses seeds germination or triggers seeds dormancy but underlying mechanism by which HT mediates seeds germination thermoinhibition needs more investigating.SOMis reported as the critical factor negatively controls light-irradiation seeds germination by altering Abscisic acid (ABA) and gibberellin acid (GA) biosynthesis. Here we found that HT acceleratesSOMexpressing through ABA signal transduction component ABI3, both ofabi3andsommutants seeds show high germination rate under HT in contrast to wild type seeds. Using ABI3 as the bait, we identified the epigenetic factor Powerdress (PWR) as the ABI3 interaction protein. Genetic and physiological analysis showed that PWR negatively control the expressing ofSOM, and overexpressing PWR enhanced, whilepwrmutant reduced, seeds germination thermotolerance. Without HT stress, PWR accelerated the histone H3 deacetylation level and H2A.Z deposition atSOMlocus, and thus suppressed ABI3-dependentSOMtranscription for seeds germination, HT stress blockPWRtranscriptional level, thus attenuated the inhibition effect of PWR onSOMexpressing, resulting into seeds germination thermoinhibition. Thus our finding propose a new function of PWR in controlling seeds germination under HT through histone acetylation modification and H2A.Z deposition.