Phosphohistidine and phospholysine phosphatase activities in the rat: potential protein-lysine and protein-histidine phosphatases?
Phosphohistidine and phospholysine phosphatase activities in the rat: potential protein-lysine and protein-histidine phosphatases?
复制标题
大鼠中的磷酸组氨酸和磷酸赖氨酸磷酸酶活性：潜在的蛋白赖氨酸和蛋白组氨酸磷酸酶？
DOI：
10.1042/bj2960293
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Kennelly,PJ
中科院分区：
文献类型：
--
作者：
Wong,C;Faiola,B;Wu,W;Kennelly,PJ
我们使用部分磷酸化的组氨酸和赖氨酸的高分子质量 (> 10 kDa) 聚合物作为底物，检测了大鼠组织提取物中的磷酸组氨酸和磷酸赖氨酸磷酸酶活性。基于观察到的热稳定性、对二价金属离子和硫醇修饰试剂的敏感性和/或从 DE-52 纤维素中洗脱的差异，这些提取物中存在多种磷酸组氨酸和磷酸赖氨酸特异性磷酸酶。这些磷酸组氨酸和磷酸赖氨酸磷酸酶的性质不同于作用于游离磷酸氨基酸磷酸精氨酸、3-磷酸组氨酸或磷酸赖氨酸的磷酸单酯特异性蛋白磷酸酶或N-P磷酸水解酶。
We have detected phosphohistidine and phospholysine phosphatase activities in rat tissue extracts using partially phosphorylated, high-molecular-mass (> 10 kDa) polymers of histidine and lysine as substrates. Multiple phosphohistidine- and phospholysine-specific phosphatases were present in these extracts based on observed differences in heat stability, sensitivity to bivalent metal ions and thiol modifying reagents, and/or elution from DE-52 cellulose. The properties of these phosphohistidine and phospholysine phosphatases were distinct from those of the phosphomonoester-specific protein phosphatases or the N-P phosphohydrolases that act on the free phosphoamino acids phosphoarginine, 3-phosphohistidine or phospholysine.