A NEW ESCHERICHIA-COLI CELL-DIVISION GENE, FTSK
A NEW ESCHERICHIA-COLI CELL-DIVISION GENE, FTSK
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DOI：
10.1128/jb.177.21.6211-6222.1995
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发表时间：
1995-11-01
影响因子：
3.2
通讯作者：
DONACHIE, WD
中科院分区：
文献类型：
--
作者：
BEGG, KJ;DEWAR, SJ;DONACHIE, WD
新发现的大肠杆菌细胞分裂基因 ftsK 中的突变会导致分裂中温度敏感的后期阻断，但不会影响染色体复制或分离。该缺陷可通过删除编码肽聚糖 DD-羧肽酶 (PBP 5) 的 dacA 来特异性抑制。FtsK 是一种大多肽 (147 kDa)，由 N 端结构域（具有多个预测的跨膜区域）、富含脯氨酸谷氨酰胺的结构域和具有核苷酸结合共有序列的 C 端结构域组成，FtsK 与来自多种原核生物的蛋白质家族具有广泛的序列同一性和质粒。质粒蛋白是细胞间 DNA 转移所必需的，并且一种细菌蛋白（枯草芽孢杆菌的 SpoIIIE 蛋白）也与细胞内染色体 DNA 转移有关。
A mutation in a newly discovered Escherichia coli cell division gene, ftsK, causes a temperature-sensitive late-stage block in division but does not affect chromosome replication or segregation. This defect is specifically suppressed by deletion of dacA, coding for the peptidoglycan DD-carboxypeptidase, PBP 5. FtsK is a large polypeptide (147 kDa) consisting of an N-terminal domain,vith several predicted membrane-spanning regions, a proline-glutamine-rich domain, and a C-terminal domain with a nucleotide-binding consensus sequence, FtsK has extensive sequence identity with a family of proteins from a wide variety of prokaryotes and plasmids. The plasmid proteins are required for intercellular DNA transfer, and one of the bacterial proteins (the SpoIIIE protein of Bacillus subtilis) has also been implicated in intracellular chromosomal DNA transfer.