Investigation of glial cells in semithin sections. I. Identification of glial cells in the brain of young rats
Investigation of glial cells in semithin sections. I. Identification of glial cells in the brain of young rats
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半薄切片中神经胶质细胞的研究。
DOI：
10.1002/cne.901490104
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发表时间：
1973
影响因子：
2.5
通讯作者：
C. P. Leblond
中科院分区：
文献类型：
--
作者：
E. Ling;J. Paterson;A. Privat;S. Mori;C. P. Leblond
用甲苯胺蓝对半微米厚的Epon切片进行染色，为识别神经胶质细胞提供了可靠的方法。在1月龄大鼠胼胝体中观察到的这些细胞的诊断特征如下：（1）星形胶质细胞具有非常苍白的细胞核和细胞质;核被膜由薄的染色质衬里清晰地勾勒出，偶尔有染色质珠;（2）小胶质细胞具有小的细胞核，其中大的深色染色质团与核质形成对比;细胞核呈圆形或细长，可能有点棱角。周细胞有一个类似但通常为新月形的核，可能与小胶质细胞有关。(3)少突胶质细胞从苍白到非常致密不等，可以任意分为三个亚群：浅色少突胶质细胞是大的苍白细胞，具有圆形至卵圆形的核，含有突出的核仁和很少或没有浓缩的染色质;细胞质广泛，看起来苍白，但不如细胞核。中等阴影的少突胶质细胞是较小的细胞，具有卵圆形核，携带小的染色质团块，整个细胞呈现中度嗜碱性;细胞质不太广泛，比“亮”亚组略暗。深色少突胶质细胞通常比中等色调的细胞小，并且通常具有带大染色质团的锯齿状或角状核;核质和细胞质都被密集染色;细胞质通常很少并且积聚在细胞核的一侧。
The staining of half‐micron thick Epon sections with toluidine blue provides a reliable method for the identification of glial cells. The diagnostic features of these cells observed in the corpus callosum of one‐month‐old rats are as follows: (1) Astrocytes have a very pale nucleus and cytoplasm; the nuclear envelope is sharply outlined by a thin chromatin lining with occasional chromatin beads; (2) Microglia have a small nucleus in which large, dark chromatin masses contrast with the nucleoplasm; the nucleus is round or elongated, and may be somewhat angular. The pericytes, which have a similar but usually crescentic nucleus, may be related to microglia. (3) Oligodendrocytes vary from pale to very dense and may be arbitrarily classified into three subgroups: The light oligodendrocytes are large pale cells with round to ovoid nucleus containing a prominent nucleolus and little or no condensed chromatin; the cytoplasm is extensive and appears pale, although less so than the nucleus. The medium shade oligodendrocytes are smaller cells that have an ovoid nucleus carrying small chromatin clumps and appearing moderately basophilic throughout; the cytoplasm is less extensive and somewhat darker than in the “light” subgroup. The dark oligodendrocytes are usually smaller than medium shade cells and often have an indented or angular nucleus with large chromatin masses; both nucleoplasm and cytoplasm are densely stained; the cytoplasm is often scanty and accumulated on one side of the nucleus.