Caffeine interferes embryonic development through over-stimulating serotonergic system in chicken embryo.
Caffeine interferes embryonic development through over-stimulating serotonergic system in chicken embryo.
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DOI：
10.1016/j.fct.2012.03.037
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发表时间：
2012-06
期刊：
影响因子：
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通讯作者：
Xiao-di Li;Rong-Rong He-Rong;Yang Qin;Bun Tsoi;Yi-Fang Li;Zheng-lai Ma;Xuesong Yang;H. Kurihara
中科院分区：
文献类型：
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作者：
Xiao-di Li;Rong-Rong He-Rong;Yang Qin;Bun Tsoi;Yi-Fang Li;Zheng-lai Ma;Xuesong Yang;H. Kurihara
咖啡因对孕妇的潜在危害引起了公众的关注，因为它可能危及胎儿的发育。单胺类神经递质被认为通过母胎相互作用调节神经发育过程，可能对精神和行为产生长期影响。本研究以鸡胚为材料，探讨咖啡因对单胺类神经递质系统的影响。咖啡因对鸡胚的毒性ED_（50）为27.3μmol/蛋。低于ED_（50）（2.5、5.0和10.0μmol/蛋）的剂量给予咖啡因可导致神经管关闭失败。10.0μmol/鸡蛋的咖啡因能增加5-HT及其代谢产物5-HIAA的含量。咖啡因处理也增加了TPH 2的基因表达。咖啡因可导致胚胎神经管闭合缺陷，神经能系统发育障碍，从而增加胚胎致畸率。同时，它也可能是导致成年人心理和行为障碍的潜在因素。此外，研究发现咖啡因会积聚在胚胎大脑中而不会被代谢，这可能会导致不良反应的放大。本研究为进一步研究咖啡因在妊娠不同阶段的毒理学作用提供了有价值的数据。
The potential harmful effects of caffeine in pregnant women aroused public interests due to its possibility to jeopardize fetal development. Monoamine neurotransmitters are thought to regulate neural development processes through maternal–fetal interactions, which may have long term impact on mental and behavioral effects. The current study focuses on investigating the effects of caffeine on the monoamine neurotransmitter system using developmental chicken embryos. The ED50value of caffeine toxicity was 27.3μmol/egg in chicken embryo. Administration of caffeine, with lower dosage than ED50(2.5, 5.0 and 10.0μmol/egg), caused failure of neural tube closure. In addition, contents of 5-HT and its metabolite 5-HIAA were increased under dosage of 10.0μmol/egg caffeine administration. Gene expression of TPH2 was also increased by caffeine treatment. Caffeine could result in defect of neural tube closure and induce disorder of serotonergic system development, which may increase teratogenic rate of embryos. Meanwhile, it is probably an underlying factor for inducing psychological and behavioral disorders in adult. Moreover, caffeine was found to be accumulated in the embryonic brain and not being metabolized, which may incur a magnification of adverse effects. This study may provide valuable data for further investigations on toxicology of caffeine during different stages of pregnancy.