CONSERVED FEATURES OF EUKARYOTIC HSP70 GENES REVEALED BY COMPARISON WITH THE NUCLEOTIDE-SEQUENCE OF HUMAN HSP70
CONSERVED FEATURES OF EUKARYOTIC HSP70 GENES REVEALED BY COMPARISON WITH THE NUCLEOTIDE-SEQUENCE OF HUMAN HSP70
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DOI：
10.1073/pnas.82.19.6455
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
MORIMOTO, RI
中科院分区：
文献类型：
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作者：
HUNT, C;MORIMOTO, RI
我们测定了人热休克蛋白70基因及其5“侧翼区的核苷酸序列。hsp 70基因转录为2440个核苷酸的不间断初级转录物，由212个核苷酸的5“非编码前导序列、242个核苷酸的3”非编码区和1986个核苷酸的连续开放阅读框组成，其编码预测分子量为69，800道尔顿的蛋白质。5“末端的上游是典型的TATAAA盒、与CCAAT基序反向对应的序列ATTGG和在14个位置中的12个与果蝇热休克基因共有的共有转录调控序列具有同源性的二联体序列CTGGAAT/ATTCCCG。将预测的人hsp 70的氨基酸序列与已发表的果蝇hsp 70和大肠杆菌dnaK的氨基酸序列进行比较，发现人hsp 70与果蝇hsp 70的同源性为73%，与大肠杆菌dnaK的同源性为47%。coli dnaK.令人惊讶的是，人类和果蝇基因的核苷酸序列有72%相同，人类和E。大肠杆菌基因有50%相同，考虑到遗传密码的简并性，这比必要的高度保守。缺乏积累的沉默核苷酸取代导致我们提出，可能有额外的信息在hsp 70基因的核苷酸序列或相应的mRNA，排除了沉默密码子位置允许的最大分歧。
We have determined the nucleotide sequence of the human hsp70 gene and 5'' flanking region. The hsp70 gene is transcribed as an uninterrupted primary transcript of 2440 nucleotides composed of a 5'' noncoding leader sequence of 212 nucleotides, a 3'' noncoding region of 242 nucleotides, and a continuous open reading frame of 1986 nucleotides that encodes a protein with predicted molecular mass of 69,800 daltons. Upstream of the 5'' terminius are the canonical TATAAA box, the sequence ATTGG that corresponds in the inverted orientation to the CCAAT motif, and the dyad sequence CTGGAAT/ATTCCCG that shares homology in 12 of 14 positions with the consensus transcription regulatory sequence common to Drosophila heat shock genes. Comparison of the predicted amino acid sequences of human hsp70 with the published sequences of Drosophila hsp70 and Escherichia coli dnaK reveals that human hsp70 is 73% identical to Drosophila hsp70 and 47% identical to E. coli dnaK. Surprisingly, the nucleotide sequences of the human and Drosophila genes are 72% identical and human and E. coli genes are 50% identical, which is more highly conserved than necessary given the degeneracy of the genetic code. The lack of accumulated silent nucleotide substitutions leads us to propose that there may be additional information in the nucleotide sequence of the hsp70 gene or the corresponding mRNA that precludes the maximum divergence allowed in the silent codon positions.