Afferent Inputs to Neurotransmitter-Defined Cell Types in the Ventral Tegmental Area.
Afferent Inputs to Neurotransmitter-Defined Cell Types in the Ventral Tegmental Area.
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DOI：
10.1016/j.celrep.2016.05.057
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发表时间：
2016-06-21
期刊：
影响因子：
8.8
通讯作者：
Hnasko TS
中科院分区：
文献类型：
--
作者：
Faget L;Osakada F;Duan J;Ressler R;Johnson AB;Proudfoot JA;Yoo JH;Callaway EM;Hnasko TS
腹侧被盖区（VTA）在行为强化的神经回路控制中起着核心作用。虽然被认为是多巴胺能神经核，但腹侧被盖区在神经递质类型上具有很大的异质性，还含有GABA和谷氨酸神经元。在这里，我们使用了一种组合病毒的方法来transsynaptically标签传入定义腹侧被盖区多巴胺，GABA，或谷氨酸神经元。令人惊讶的是，我们发现，这些人群收到了定性相似的输入，从外侧下丘脑，中缝，和腹侧苍白球占主导地位的和可比的预测。然而，观察到显着的差异与纹状体区和苍白球提供更大份额的输入腹侧被盖区多巴胺神经元，皮层输入优先谷氨酸神经元，和GABA神经元接收比例更多的输入从外侧缰核和背外侧被盖核。通过比较每个发射机定义的VTA细胞类型的输入，这项研究揭示了重要的光VTA的不同输入的系统级组织。腹侧被盖区（VTA）神经元的传入控制奖赏寻求行为，但它们在异质VTA细胞类型上的相对排列仍是未知的。Faget等人现在使用一种优化的跨突触逆行追踪方法，为每个发射器定义的VTA细胞类型提供全面的输入映射。
The ventral tegmental area (VTA) plays a central role in the neural circuit control of behavioral reinforcement. Though considered a dopaminergic nucleus, the VTA contains substantial heterogeneity in neurotransmitter type, containing also GABA and glutamate neurons. Here we used a combinatorial viral approach to transsynaptically label afferents to defined VTA dopamine, GABA, or glutamate neurons. Surprisingly, we find that these populations received qualitatively similar inputs, with dominant and comparable projections from the lateral hypothalamus, raphe, and ventral pallidum. However, notable differences were observed with striatal regions and globus pallidus providing a greater share of input to VTA dopamine neurons, cortical input preferentially on to glutamate neurons, and GABA neurons receiving proportionally more input from the lateral habenula and laterodorsal tegmental nucleus. By comparing inputs to each of the transmitter-defined VTA cell types this study sheds important light on the systems-level organization of diverse inputs to VTA. Afferents to ventral tegmental area (VTA) neurons control reward-seeking behaviors, but their relative arrangement onto heterogeneous VTA cell types has remained largely unknown. Faget et al. now use an optimized transsynaptic retrograde tracing approach to provide a comprehensive map of inputs to each transmitter-defined VTA cell type.