Comparative analysis of remyelinating potential of focal and intravenous administration of autologous bone marrow cells into the rat demyelinated spinal cord
Comparative analysis of remyelinating potential of focal and intravenous administration of autologous bone marrow cells into the rat demyelinated spinal cord
复制标题
DOI：
10.1002/glia.10285
复制
发表时间：
2003-11-01
期刊：
影响因子：
6.2
通讯作者：
Kocsis, JD
中科院分区：
文献类型：
--
作者：
Inoue, M;Honmou, O;Kocsis, JD
在直接注射和静脉注射到脊髓脱髓鞘病变的大鼠后，研究了自体骨髓细胞的髓鞘再生潜力。局灶性和静脉注射急性分离的单核骨髓细胞组分导致不同程度的髓鞘再生。以不同浓度（直接注射10（2）-10（5），静脉注射10（4）-10（7））递送从同一大鼠采集的骨髓细胞混悬液。移植后3周行组织学检查。光学显微镜检查显示，两种注射方案均出现背索髓鞘再生，但髓鞘再生的程度与注射细胞的数量成正比。为了获得通过直接注射实现的相同的髓鞘再生的相对密度，静脉内施用细胞需要递送实质上更多的细胞（两个数量级）。然而，大量自体骨髓细胞的可用性和在没有神经外科干预的情况下将细胞全身递送至靶病变区域的潜力表明静脉内细胞递送作为脱髓鞘疾病的前瞻性治疗方法的潜在效用。(C)2003威利利斯公司
The remyelinating potential of autologous bone marrow cells was studied after direct injection and following intravenous injection into rats with a demyelinated lesion in the spinal cord. Both focal and intravenous injections of acutely isolated mononuclear bone marrow cell fractions resulted in varying degrees of remyelination. Suspensions of bone marrow cells collected from the same rat were delivered at varied concentrations (10(2) to 10(5) for direct injection and 10(4) to 10(7) for i.v. injections). The lesions were examined histologically 3 weeks after transplantation. Light microscopic examination revealed remyelination in the dorsal funiculus with both injection protocols, but the extent of remyelination was proportional to the number of injected cells. To attain the same relative density of remyelination achieved by direct injection, intravenous administration of cells required delivery of substantially more cells (two orders of magnitude). However, the availability of autologous bone marrow cells in large number and the potential for systemically delivering cells to target lesion areas without neurosurgical intervention suggest the potential utility of intravenous cell delivery as a prospective therapeutic approach in demyelinating disease. (C) 2003 Wiley-Liss, Inc.