Branched polymeric labels used as drag-tags in free-solution electrophoresis of ssDNA.
Branched polymeric labels used as drag-tags in free-solution electrophoresis of ssDNA.
复制标题
在 ssDNA 自由溶液电泳中用作拖曳标签的支链聚合物标签。
DOI：
10.1002/elps.200500471
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Slater,GaryW
中科院分区：
文献类型：
--
作者：
Nedelcu,Sorin;Slater,GaryW
In the framework of the classical blob theory of end‐labeled free‐solution electrophoresis of ssDNA, and based on recent experimental data with linear and branched polymeric labels (or drag‐tags), the present study puts forward design principles for the optimal type of branching that would give, for a given total number of monomers, the highest effective frictional drag for ssDNA sequencing purposes. The hydrodynamic radii of the linear and branched labels are calculated using standard models like the freely jointed chain model and the Kratky–Porod worm‐like chain model. Based on comparisons of the theory with the experimental data, we propose that the design of new branched labels should use either side chains whose length is comparable to the distance between the branching points or two long branches located near the ends of the molecule's backbone.
