Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study.
Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study.
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DOI：
10.1016/s1473-3099(13)70164-6
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发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Koopmans MP
中科院分区：
文献类型：
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作者：
Reusken CB;Haagmans BL;Müller MA;Gutierrez C;Godeke GJ;Meyer B;Muth D;Raj VS;Smits-De Vries L;Corman VM;Drexler JF;Smits SL;El Tahir YE;De Sousa R;van Beek J;Nowotny N;van Maanen K;Hidalgo-Hermoso E;Bosch BJ;Rottier P;Osterhaus A;Gortázar-Schmidt C;Drosten C;Koopmans MP
在严重急性呼吸道感染患者中发现了一种新的β冠状病毒-中东呼吸综合征冠状病毒（MERS-CoV）。虽然相关病毒感染蝙蝠，但分子钟分析无法确定MERS-CoV的直接祖先。轶事接触史表明，患者曾与单峰骆驼或山羊接触。我们通过评估牲畜中的特异性血清抗体来调查MERS CoV的可能动物储库。我们从中东（阿曼）和其他地方（西班牙，荷兰，智利）的动物身上提取血清。牛（n=80），绵羊（n=40），山羊（n = 40），单峰骆驼（n=155），和各种其他骆驼科物种（n=34）进行了测试特异性血清IgG的蛋白质微阵列使用受体结合S1亚基的刺突蛋白MERS冠状病毒，严重急性呼吸综合征冠状病毒，和人冠状病毒OC 43。MERS CoV和牛冠状病毒的病毒中和试验证实了结果。来自阿曼骆驼的50份血清中的50份（100%）和来自西班牙骆驼的105份血清中的15份（14%）具有针对MERS-CoV刺突的蛋白特异性抗体。来自欧洲的绵羊、山羊、牛和其他骆驼科动物的血清没有这种抗体。MERS-CoV中和抗体滴度在阿曼骆驼血清的1/320和1/2560之间变化，在西班牙骆驼血清的1/20和1/320之间变化。没有证据表明牛冠状病毒抗体具有交叉中和作用。MERS-CoV或相关病毒已感染骆驼种群。来自阿曼不同地点的血清中的滴度和血清阳性率均表明存在广泛感染。欧洲联盟、欧洲疾病预防和控制中心、德国研究共同体。
A new betacoronavirus—Middle East respiratory syndrome coronavirus (MERS-CoV)—has been identified in patients with severe acute respiratory infection. Although related viruses infect bats, molecular clock analyses have been unable to identify direct ancestors of MERS-CoV. Anecdotal exposure histories suggest that patients had been in contact with dromedary camels or goats. We investigated possible animal reservoirs of MERS-CoV by assessing specific serum antibodies in livestock. We took sera from animals in the Middle East (Oman) and from elsewhere (Spain, Netherlands, Chile). Cattle (n=80), sheep (n=40), goats (n=40), dromedary camels (n=155), and various other camelid species (n=34) were tested for specific serum IgG by protein microarray using the receptor-binding S1 subunits of spike proteins of MERS-CoV, severe acute respiratory syndrome coronavirus, and human coronavirus OC43. Results were confirmed by virus neutralisation tests for MERS-CoV and bovine coronavirus. 50 of 50 (100%) sera from Omani camels and 15 of 105 (14%) from Spanish camels had protein-specific antibodies against MERS-CoV spike. Sera from European sheep, goats, cattle, and other camelids had no such antibodies. MERS-CoV neutralising antibody titres varied between 1/320 and 1/2560 for the Omani camel sera and between 1/20 and 1/320 for the Spanish camel sera. There was no evidence for cross-neutralisation by bovine coronavirus antibodies. MERS-CoV or a related virus has infected camel populations. Both titres and seroprevalences in sera from different locations in Oman suggest widespread infection. European Union, European Centre For Disease Prevention and Control, Deutsche Forschungsgemeinschaft.