INCREASE IN CELL MASS DURING THE DIVISION CYCLE OF ESCHERICHIA-COLI B/RA
INCREASE IN CELL MASS DURING THE DIVISION CYCLE OF ESCHERICHIA-COLI B/RA
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DOI：
10.1128/jb.168.2.613-618.1986
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发表时间：
1986-11-01
影响因子：
3.2
通讯作者：
KUBITSCHEK, HE
中科院分区：
文献类型：
--
作者：
KUBITSCHEK, HE
在大肠杆菌B/rA的分裂周期中，未分裂细胞的平均细胞质量增加。细胞在分裂周期中的浮力密度是在指数生长培养的细胞按大小分离后测定的。这些细胞的浮力密度基本上与细胞年龄无关，平均值为1.094 g ml-1。在两种不同的培养基中同步生长时，平均细胞体积和浮力密度也被测定，这两种培养基提供了40和25分钟的加倍时间。细胞体积和质量在两种生长速率下均呈线性增长，而浮力密度变化不显著。结果与细胞生长的三种主要模型中只有一种是一致的，即线性生长，它规定细胞质量的增长率在整个分裂周期中是恒定的。
Increase in the mean cell mass of undivided cells was determined during the division cycle of Escherichia coli B/rA. Cell buoyant densities during the division cycle were determined after cells from an exponentially growing culture were separated by size. The buoyant densities of these cells were essentially independent of cell age, with a mean value of 1.094 g ml-1. Mean cell volume and buoyant density were also determined during synchronous growth in two different media, which provided doubling times of 40 and 25 min. Cell volume and mass increased linearly at both growth rates, as buoyant density did not vary significantly. The results are consistent with only one of the three major models of cell growth, linear growth, which specifies that the rate of increase in cell mass is constant throughout the division cycle.