SARS-CoV-2 on Surfaces and HVAC Filters in Dormitory Rooms
SARS-CoV-2 on Surfaces and HVAC Filters in Dormitory Rooms
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DOI：
10.1021/acs.estlett.1c00892
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发表时间：
2021-12-17
影响因子：
10.9
通讯作者：
Marr, Linsey C.
中科院分区：
文献类型：
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作者：
Pan, Jin;Hawks, Seth A.;Marr, Linsey C.
虽然已经有许多关于医院病房中SARS-CoV2污染的研究，但对病毒在非医疗保健环境中的存在知之甚少，而非医疗保健环境是传播最多的地方。我们调查了隔离或隔离学生宿舍中的病毒污染情况。采集24个新冠肺炎阳性学生居住的房间的表面拭子和暖通空调过滤器样本，采用定量逆转录聚合酶链式反应(RT-qPCR)检测病毒核酸。两个宿舍共有15/21(71.4%)的暖通空调过滤器、71/125(56.8%)的表面样本和4/6(66.7%)的浴室排烟格栅检测到病毒RNA。病毒RNA存在于所有五种表面样本中，包括水槽把手、水槽台面、水槽附近的地板、门把手和恒温器面板。病毒表面的RNA水平变化很大，从10到10(4)个基因拷贝/拭子区域，类似于10厘米×10厘米。此外，我们还检测了Ct值低于33的样品的传染性，没有一份样品呈阳性。这些信息将对评估新冠肺炎通过空气和病毒传播的风险很有价值。
While there have been many studies of SARS-CoV2 contamination in hospital patients' rooms, less is known about the virus' presence in nonhealthcare environments, which is where most transmission takes place. We investigated virus contamination in university dormitories housing students who were in quarantine or isolation. We collected surface swab samples and heating, ventilation, and air conditioning (HVAC) filters from 24 rooms that had been occupied by students who tested positive for COVID-19, and we measured viral RNA by the quantitative reverse transcription polymerase chain reaction (RT-qPCR). We detected viral RNA on or in 15/21 (71.4%) HVAC filters, 71/125 (56.8%) surface samples, and 4/6 (66.7%) bathroom exhaust grilles in the two dormitories combined. Viral RNA was present in all five types of surface samples, including sink handles, sink countertops, floors near the sink, door handles, and thermostat panels. Viral RNA levels on surfaces varied widely, from 10 to >10(4) gene copies per swabbed area of similar to 10 cm x 10 cm. Additionally, we tested the infectivity of samples with a Ct value lower than 33, and none of them were positive. This information will be valuable for assessing the risk of airborne and fomite transmission of COVID-19.