VIRAL PATHOGENESIS OF A NEPHROTROPIC INFECTIOUS-BRONCHITIS VIRUS ISOLATED FROM COMMERCIAL PULLETS
VIRAL PATHOGENESIS OF A NEPHROTROPIC INFECTIOUS-BRONCHITIS VIRUS ISOLATED FROM COMMERCIAL PULLETS
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DOI：
10.2307/1591200
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发表时间：
1991-04-01
期刊：
影响因子：
1.4
通讯作者：
REYNOLDS, B
中科院分区：
文献类型：
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作者：
KINDE, H;DAFT, BM;REYNOLDS, B
传染性支气管炎是在3至4周大的小鸡中被诊断出来的，这些小鸡来自加利福尼亚的一个商业禽群。该疾病的特征是头部肿胀，眼睛和鼻孔有水状分泌物，肾脏有尿酸。死亡率从每周1.8%到12.5%不等。从10日龄鸡胚第5代临床病鸡肾池悬浊液中分离到一种冠状病毒，并对肾脏病变进行了肉眼和组织学检查，并对接种的鸡进行了酶联免疫吸附试验，结果表明分离到的病毒是一种传染性支气管炎病毒（IBV）肾营养毒株。根据在鸡胚蛋中进行的病毒中和试验，发现该病毒分离物是一种以前未被识别的血清型。通过将IBV分离株接种到无特定病原体的雏鸡体内并观察肾脏病变，证实了IBV分离株的肾致病性。在加利福尼亚，以前没有从家禽中分离出嗜肾性IBV菌株的报道。
Infectious bronchitis was diagnosed in 3-to-4-week-old pullets from an out-break in a commercial flock in California. The disease was characterized by head swelling, watery discharge from the eyes and nostrils, and urates in kidneys. Mortality ranged from 1.8% to 12.5% per week. The isolation of a coronavirus from a suspension of pooled kidneys from clinically ill chickens at the fifth passage in 10-day-old chicken embryos, gross and histologic renal lesions, and seroconversion by enzyme-linked immunosorbent assay in inoculated birds suggested that the virus isolated was a nephrotrophic strain of infectious bronchitis virus (IBV). The virus isolate was found to be a previously unrecognized serotype, based on virus neutralization tests performed in embryonated chicken eggs. Nephropathogenicity of the IBV isolate was confirmed by inoculation of the viral isolate into specific-pathogen-free chicks and demonstration of renal lesions. The isolation of nephrotropic strains of IBV has not been reported previously from poultry in California.