Expression of cytoskeletal protein 4.1 during avian erythroid cellular maturation.
Expression of cytoskeletal protein 4.1 during avian erythroid cellular maturation.
复制标题
禽类红细胞成熟过程中细胞骨架蛋白4.1的表达。
DOI：
10.1073/pnas.84.4.1035
复制
发表时间：
1987
影响因子：
11.1
通讯作者：
Engel,JD
中科院分区：
文献类型：
--
作者：
Yew,NS;Choi,HR;Gallarda,JL;Engel,JD
我们分离出了编码蛋白质 4.1 部分的 cDNA 克隆，蛋白质 4.1 是红细胞细胞骨架的组成部分。使用针对蛋白 4.1 的单克隆抗体，通过对鸡红细胞 lambda gt11 cDNA 文库进行免疫学筛选来分离重组体。 DNA 印迹分析显示该基因在每个单倍体鸡基因组中以单个拷贝的形式存在，而 RNA 印迹分析显示网织红细胞中存在 7 KB 的单个 mRNA。相同大小的信息（数量减少）也存在于由禽类成红细胞增多症病毒转化的红白血病细胞系中，并且在非红系造血细胞中的数量也大大减少。免疫印迹和免疫荧光实验表明，鸡4.1变异蛋白的一个子集在鸡红白血病细胞的体外分化过程中优先表达。这些数据表明该基因在早期红系细胞成熟过程中活跃地转录和翻译。
We have isolated a cDNA clone encoding part of protein 4.1, an integral component of the erythrocyte cytoskeleton. The recombinant was isolated by immunological screening of a chicken erythroid lambda gt11 cDNA library using a monoclonal antibody directed against protein 4.1. DNA blot analysis shows that the gene is present as a single copy per haploid chicken genome, while RNA blot analysis reveals the presence of a single mRNA of 7 kilobases in reticulocytes. Message of the same size (in reduced amounts) is also present in an erythroleukemic cell line transformed by avian erythroblastosis virus and is also present in vastly reduced quantities in nonerythroid hemopoietic cells. Immunoblotting and immunofluorescence experiments show that a subset of the chicken 4.1 variant proteins is preferentially expressed during in vitro differentiation of chicken erythroleukemic cells. These data indicate that the gene is both actively transcribed and translated during early erythroid cellular maturation.