Phase coherent excitation of SABRE permits simultaneous hyperpolarization of multiple targets at high magnetic field
Phase coherent excitation of SABRE permits simultaneous hyperpolarization of multiple targets at high magnetic field
复制标题
SABRE 的相位相干激励允许在高磁场下同时超极化多个目标
DOI：
10.1039/d1cp05962a
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发表时间：
2022
影响因子：
3.3
通讯作者：
Warren, Warren S.
中科院分区：
文献类型：
--
作者：
Lindale, Jacob R.;Eriksson, Shannon L.;Warren, Warren S.
磁共振中的超极化方法克服了灵敏度限制，特别是对低γ核，如13C和15N。可逆交换信号放大(SABRE)和扩展SABRE(X-SABRE)是在各种原子核上产生大极化的有效且低成本的方法，但它们最常用的是低磁场(μT-mT)。直接在光谱仪中产生超极化的高场方法可能要方便得多，但仅限于选择性地使单个目标超极化。在这里，我们介绍了一种新的基于脉冲序列的方法，该方法在强磁场下提供了SABRE超极化的宽带激励，而不必针对特定的目标定制脉冲序列参数。这首次允许多个目标在高场下同时超极化，并提供了一种将高场SABRE超极化集成到常规核磁共振应用中的直接方法，例如基于核磁共振的代谢组学和生物分子核磁共振。
Hyperpolarization methods in magnetic resonance overcome sensitivity limitations, especially for low-γ nuclei such as 13C and 15N. Signal Amplification By Reversible Exchange (SABRE) and extended SABRE (X-SABRE) are efficient and low-cost methods for generating large polarizations on a variety of nuclei, but they most commonly use low magnetic fields (μT–mT). High field approaches, where hyperpolarization is generated directly in the spectrometer, are potentially much more convenient but have been limited to selectively hyperpolarize single targets. Here we introduce a new pulse sequence-based approach that affords broadband excitation of SABRE hyperpolarization at high magnetic fields without having to tailor pulse sequence parameters to specific targets. This permits simultaneous hyperpolarization of multiple targets for the first time at high field and offers a direct approach to integration of high-field SABRE hyperpolarization into routine NMR applications, such as NMR-based metabonomics and biomolecular NMR.