Differential Postsynaptic Compartments in the Laterocapsular Division of the Central Nucleus of Amygdala for Afferents From the Parabrachial Nucleus and the Basolateral Nucleus in the Rat
Differential Postsynaptic Compartments in the Laterocapsular Division of the Central Nucleus of Amygdala for Afferents From the Parabrachial Nucleus and the Basolateral Nucleus in the Rat
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DOI：
10.1002/cne.22487
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发表时间：
2010-12-01
影响因子：
2.5
通讯作者：
Shigemoto, Ryuichi
中科院分区：
文献类型：
--
作者：
Dong, Yu-Lin;Fukazawa, Yugo;Shigemoto, Ryuichi
杏仁核中央核 (CeC) 的后囊区神经元被称为伤害性杏仁核，接收来自臂旁核 (PB) 和杏仁核基底外侧核 (BLA) 的谷氨酸输入，分别传递来自脊髓背角的伤害性信息以及来自丘脑和皮质的多模态信息。使用表达 EGFP 的慢病毒在大鼠中鉴定的 PB- 和 BLA-CeC 突触的超微结构特性 此外，通过使用高灵敏度 SDS 消化的冷冻断裂复制品标记 (SDS-FRL) 研究了 CeC 神经元上突触 AMPA 受体 (AMPAR) 的密度。来自 PB 的传入信号主要在树突轴上形成不对称突触 (88%)，而来自 BLA 的传入信号则在树突棘上(81%) 树突轴中的 PB-CeC 突触明显大于棘中的 BLA-CeC 突触（中位数 0 072 μ m(2)）（中位数 0 058 μ m(2)，P = 0 02） 用 PB 纤维形成突触的树突轴也显着大于用 BLA 纤维形成突触的树突轴，表明 PB 纤维在更近端形成突触SDS-FRL 显示，几乎所有兴奋性突触后位点在 CeC 中都有 AMPAR。单个突触中 AMPAR 特异性金颗粒的密度在棘突触中显着高于轴突触（中位数 510 个颗粒/μ m(2)，P = 0·01）。这些结果表明，PB- 和 PB- 的不同突触影响BLA-CeC 通路有助于整合 CeC 中的伤害感受和多模态信息 J Comp Neurol 518 47714791, 2010 (C) 2010 Wiley Liss Inc
Neurons in the laterocapsular division of the central nucleus of the amygdala (CeC), which is known as the nociceptive amygdala,' receive glutamatergic inputs from the parabrachial nucleus (PB) and the basolateral nucleus of amygdala (BLA), which convey nociceptive information from the dorsal horn of the spinal cord and polymodal information from the thalamus and cortex, respectively Here, we examined the ultrastructural properties of PB- and BLA-CeC synapses identified with EGFP-expressing lentivirus in rats In addition, the density of synaptic AMPA receptors (AMPARs) on CeC neurons was studied by using highly sensitive SDS-digested freeze-fracture replica labeling (SDS-FRL) Afferents from the PB made asymmetrical synapses mainly on dendritic shafts (88%), whereas those from the BLA were on dendritic spines (81%) PB-CeC synapses in dendritic shafts were significantly larger (median 0 072 mu m(2)) than BLA-CeC synapses in spines (median 0 058 mu m(2), P = 0 02) The dendritic shafts that made synapses with PB fibers were also significantly larger than those that made synapses with BLA fibers, indicating that the PB fibers make synapses on more proximal parts of dendrites than the BLA fibers SDS-FRL revealed that almost all excitatory postsynaptic sites have AMPARs in the CeC The density of AMPAR-specific gold particles in individual synapses was significantly higher in spine synapses (median 510 particles/mu m(2)) than in shaft synapses (median 427 particles/mu m(2), P = 0 01) These results suggest that distinct synaptic impacts from PB- and BLA-CeC pathways contribute to the integration of nociceptive and polymodal information in the CeC J Comp Neurol 518 47714791, 2010 (C) 2010 Wiley Liss Inc