DYE-MEDIATED PHOTOLYSIS OF NORMAL AND NEOPLASTIC HEMATOPOIETIC-CELLS
DYE-MEDIATED PHOTOLYSIS OF NORMAL AND NEOPLASTIC HEMATOPOIETIC-CELLS
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DOI：
10.1016/0145-2126(87)90104-4
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发表时间：
1987-01-01
影响因子：
2.7
通讯作者：
SENSENBRENNER, LL
中科院分区：
文献类型：
--
作者：
SIEBER, F;STUART, RK;SENSENBRENNER, LL
本研究的目的是测定正常人造血祖细胞和4种白血病细胞系（Daudi、Raji、K562和HL-60）对merocyanine 540 （mc540）介导的光解作用的敏感性。晚期红系祖细胞是最敏感的正常细胞。早期红系祖细胞具有中等敏感性。粒细胞/巨噬细胞祖细胞和多能祖细胞是最不敏感的正常骨髓细胞。染料浓度、血清浓度和光照的组合消除了50%的多能祖细胞，使白血病细胞的浓度降低了0.75%。4.5日志。可以想象，这种光敏性的差异可以用于自体缓解性骨髓移植的体外清除。
The purpose of this study was to determine the sensitivity to merocyanine 540 (MC 540)-mediated photolysis of normal human hematopoietic progenitor cells and four leukemia cell lines (Daudi, Raji, K562 and HL-60). Late erythroid progenitors were the most sensitive normal cells. Early erythroid progenitors were of intermediate sensitivity. Granulocyte/macrophage progenitors and multipotent progenitors were the least sensitive normal marrow cells. A combination of dye concentration, serum concentration, and illumination that eliminated 50% of multipotent progenitor cells reduced the concentration of leukemic cells by .gtoreq. 4.5 log. It is conceivable that this difference in photosensitivity can be exploited for the extracorporeal purging of autologous remission marrow grafts.