Determining the fate of seeded cells in venous tissue-engineered vascular grafts using serial MRI
Determining the fate of seeded cells in venous tissue-engineered vascular grafts using serial MRI
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DOI：
10.1096/fj.11-185140
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发表时间：
2011-12-01
期刊：
影响因子：
4.8
通讯作者：
Breuer, Christopher K.
中科院分区：
文献类型：
--
作者：
Harrington, Jamie K.;Chahboune, Halima;Breuer, Christopher K.
组织工程研究的一个主要限制是缺乏用于观察单个组织工程结构动态变化的非侵入性监测技术。我们使用细胞磁共振成像 (MRI) 通过连续成像来追踪接种到功能性组织工程血管移植物 (TEVG) 上的细胞的命运。经过体外优化后，用超小超顺磁性氧化铁 (USPIO) 纳米颗粒标记小鼠巨噬细胞，并将其接种到支架上，通过手术将其作为下腔静脉插入移植物植入 SCID/bg 小鼠中。连续 MRI 显示，植入 USPIO 标记支架后，横向弛豫时间 (T-2) 显着降低（T-2=44 +/- 6.8 与 71 +/- 10.2 毫秒），但在 2 小时后迅速增加至与接种未标记巨噬细胞的对照植入物相同的值（T-2=63 +/- 12 与 63 +/- 14 毫秒）。这强烈表明支架上的种子细胞迅速丢失，这一发现使用普鲁士蓝染色对外植的 TEVG 上的含铁巨噬细胞进行了验证。我们的结果支持了一种新的范例，即种子细胞从植入的支架中迅速丢失，而不是像传统认为的那样发育成新血管细胞。我们的研究结果证实并验证了这种范式转变，同时证明了无创 MRI 在组织工程中细胞水平过程的连续研究中的首次成功应用。-Harrington, J. K.、Chahboune, H.、Criscione, J. M.、Li, A. Y.、Hibino, N.、Yi, T.、Villalona, G. A.、Kobsa, S.、Meijas, D.、Duncan, D. R.、Devine, L., Papademetri，X.，Shin'oka，T.，Fahmy，T.M.，Breuer，C.K.使用连续 MRI 确定静脉组织工程血管移植物中种子细胞的命运。 FASEB J. 25, 4150-4161 (2011)。 www.fasebj.org
A major limitation of tissue engineering research is the lack of noninvasive monitoring techniques for observations of dynamic changes in single tissue-engineered constructs. We use cellular magnetic resonance imaging (MRI) to track the fate of cells seeded onto functional tissue-engineered vascular grafts (TEVGs) through serial imaging. After in vitro optimization, murine macrophages were labeled with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles and seeded onto scaffolds that were surgically implanted as inferior vena cava interposition grafts in SCID/bg mice. Serial MRI showed the transverse relaxation times (T-2) were significantly lower immediately following implantation of USPIO-labeled scaffolds (T-2=44 +/- 6.8 vs. 71 +/- 10.2 ms) but increased rapidly at 2 h to values identical to control implants seeded with unlabeled macrophages (T-2=63 +/- 12 vs. 63 +/- 14 ms). This strongly indicates the rapid loss of seeded cells from the scaffolds, a finding verified using Prussian blue staining for iron containing macrophages on explanted TEVGs. Our results support a novel paradigm where seeded cells are rapidly lost from implanted scaffolds instead of developing into cells of the neovessel, as traditionally thought. Our findings confirm and validate this paradigm shift while demonstrating the first successful application of noninvasive MRI for serial study of cellular-level processes in tissue engineering.-Harrington, J. K., Chahboune, H., Criscione, J. M., Li, A. Y., Hibino, N., Yi, T., Villalona, G. A., Kobsa, S., Meijas, D., Duncan, D. R., Devine, L., Papademetri, X., Shin'oka, T., Fahmy, T. M., Breuer, C. K. Determining the fate of seeded cells in venous tissue engineered vascular grafts using serial MRI. FASEB J. 25, 4150-4161 (2011). www.fasebj.org