The Necessity of OCT-4 and CDX2 for Early Development and Gene Expression Involved in Differentiation of Inner Cell Mass and Trophectoderm Lineages in Bovine Embryos
The Necessity of OCT-4 and CDX2 for Early Development and Gene Expression Involved in Differentiation of Inner Cell Mass and Trophectoderm Lineages in Bovine Embryos
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DOI：
10.1089/cell.2015.0081
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发表时间：
2016-10-01
影响因子：
1.6
通讯作者：
Sawai, Ken
中科院分区：
文献类型：
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作者：
Sakurai, Nobuyuki;Takahashi, Kazuki;Sawai, Ken
详细介绍了POU 5类转录因子1(Oct-4)和尾型同源框2(CDX2)在小鼠内细胞团(ICM)和滋养外胚层(TE)分化中的作用。然而，对Oct-4和CDX2在牛着床前胚胎中的作用知之甚少。为了阐明它们在牛胚胎早期发育中的功能，我们利用RNA干扰技术下调了Oct-4和CDX2的表达。我们将Oct-4或CDX2特异的短干扰RNA(SiRNAs)注入牛受精卵。与未注射或未注射siRNA的胚胎相比，Oct-4下调的胚胎的囊胚发育率较低。基因表达分析显示，在Oct-4下调的胚胎中，CDX2和成纤维细胞生长因子4的表达降低。CDX2表达下调的胚胎发育到囊胚期；然而，在大多数情况下，囊胚腔的形成被推迟。基因表达分析显示，在CDX2下调的胚胎中，GATA3的表达降低，NANOG的表达增加。综上所述，Oct-4和CDX2在牛胚胎早期发育和参与牛胚胎ICM和TE系分化的基因表达中是必不可少的。
The functions of POU class 5 transcription factor 1 (Oct-4) and caudal-type homeobox 2 (Cdx2) in the differentiation of the murine inner cell mass (ICM) and trophectoderm (TE) have been described in detail. However, little is known about the roles of OCT-4 and CDX2 in preimplantation bovine embryos. To elucidate their functions during early development in bovine embryos, we performed OCT-4 and CDX2 downregulation using RNA interference. We injected OCT-4-or CDX2-specific short interfering RNAs (siRNAs) into bovine zygotes. The rate of blastocyst development of OCT-4-downregulated embryos was lower compared with uninjected or control siRNA-injected embryos. Gene expression analysis revealed decreased CDX2 and fibroblast growth factor 4 expression in OCT-4-downregulated embryos. CDX2-downregulated embryos developed to the blastocyst stage; however, in most cases, blastocoel formation was delayed. Gene expression analysis revealed decreased GATA3 expression and elevated NANOG expression in CDX2-downregulated embryos. In conclusion, OCT-4 and CDX2 are essential for early development and gene expression involved in differentiation of ICM and TE lineages in bovine embryos.