Cloning of a MADS box gene (GhMADS3) from cotton and analysis of its homeotic role in transgenic tobacco
Cloning of a MADS box gene (GhMADS3) from cotton and analysis of its homeotic role in transgenic tobacco
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DOI：
10.1016/s1673-8527(07)60058-7
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发表时间：
2007-06-01
影响因子：
5.9
通讯作者：
Li, Mingyang
中科院分区：
文献类型：
--
作者：
Guo, Yulong;Zhu, Qinlong;Li, Mingyang
从棉花（Gossypium hirsuturn L.）基于EST序列。预测的GhMADS 3蛋白质序列分别与可可TcAG、金鱼草FAR和拟南芥AG的同源性分别为85%、73%和62%，并且当比较不包括N-延伸的全长序列时，与AG同源物分组。GhMADS 3在野生型棉花花中主要在雄蕊和心皮中表达，这与拟南芥中的AG相当。然而，在同源异型棉花变异体chv 1的花蕾中不表达。GhMADS 3在烟草中的异位表达导致花的萼片到心皮和花瓣到雄蕊的转化。心皮状的第一轮器官，其上缘有柱头组织，与野生型萼片的深绿色相比，其外观为白色。有时，在第一个心皮状橙的融合部位观察到长的细丝。退化雄蕊的第二轮器官通常比野生型小，颜色由粉红色变为白色。这些结果表明GhMADS 3在花发育中具有同源异型作用。
A MADS box gene (GhMADS3) was cloned from cotton (Gossypium hirsuturn L.) based on EST sequences. The predicted protein sequence of GhMADS3 showed 85%, 73%, and 62% identity with Theobroma cacao TcAG, Antirrhinum majus FAR, and Arabidopsis thaliana AG, respectively, and was grouped with AG homologues when the full length sequences excluding N-extensions were compared. GhMADS3 expressed in the wild type cotton flower primarily in stamens and carpels, which was comparable to AG in Arabidopsis. However, it was not expressed in floral buds of a homeotic cotton variant chv1. Ectopic expression of GhMADS3 in tobacco (Nicotiana tabacum L.) resulted in flowers with sepal-to-carpel and petal-to-stamen transformation. The carpelloid first whorl organs, with stigmatic tissue on their upper edges, had a white appearance when compared with the dark green color of the wild type sepals. At times, long filaments were observed at the fusion site of the first carpelloid oranges. The second whorl organs in staminoid were usually smaller than the wild type and the color was changed from pink to white. These results suggest that GhMADS3 has a homeotic role in flower development.