Histopathological characteristics of hypervascular cholangiocellular carcinoma as an early stage of cholangiocellular carcinoma.
Histopathological characteristics of hypervascular cholangiocellular carcinoma as an early stage of cholangiocellular carcinoma.
复制标题
富血管性胆管细胞癌作为胆管细胞癌早期阶段的组织病理学特征。
DOI：
10.1111/hepr.12236
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Shimada
中科院分区：
文献类型：
--
作者：
Sato Y;Ojima H;Onaya H;Mori T;Hiraoka N;Kishi Y;Nara S;Esaki M;Shimada
目的高血供胆管细胞癌(h-CCC)的预后优于普通低血管胆管细胞癌(o-CCC)。方法根据增强CT动脉期表现，将16例肿块型CC分为两组(h-CC，n= 8；o-CC，n= 8)。H-CCC的高密度区(H-a区)和低密度区(H-b区)和o-CCC的低密度区(O区)被勾画出来。组织病理学检查肿瘤细胞比例、纤维间质、动脉血管密度、血管内皮生长因子、血管生成素-2、细胞角蛋白7、CK19、SOX9和SOX17基因的免疫组织化学表达；结果肿瘤细胞的面积比由大到小依次为：H-a区、H-b区、O区。血管内皮细胞生长因子和血管生成素-2的表达在H-a区、H-b区、O区显著高于O区。H-b区和O区。结论h-CCC肿瘤细胞和AVD的面积比高，干细胞和血管生成标记物的表达高，而纤维间质和恶性潜能的面积比低。这些结果提示h-CCC可能代表了CCC的早期阶段。
AimPrognosis of hypervascular cholangiocellular carcinoma (h‐CCC) is reportedly better than that of ordinary hypovascular CCC (o‐CCC). The aim of this study is to clarify the histopathological characteristics of h‐CCC.MethodsOn the basis of the findings in the arterial phase of contrast‐enhanced computed tomography, 16 cases of mass‐forming‐type CCC were divided into two groups (h‐CCC,n= 8; o‐CCC,n= 8). Areas of high (Area H‐a) and low (Area H‐b) attenuation in h‐CCC cases and areas of low attenuation in o‐CCC cases (Area O) were delineated. These areas were then evaluated histopathologically to determine the proportion of tumor cells, fibrous stroma, arterial vessel density, and immunohistochemical expression of Vascular endothelial growth factor; angiopoietin‐2; cytokeratin 7, CK19,SOX9andSOX17genes; epithelial cell adhesion molecule; and the Bmi‐1, Ki‐67, epithelial membrane antigen and polyclonal carcinoembryonic antigen.ResultsThe areal ratio of tumor cells decreased and that of fibrous stroma increased in the following order: Area H‐a, Area H‐b and Area O. Values for AVD and neural cell adhesion molecule positivity rate were significantly higher in Area H‐a than in Areas H‐b or O. Expressions of vascular endothelial growth factor and angiopoietin‐2 were significantly higher in Areas H‐a and H‐b than in Area O. The Ki‐67 labeling index increased in the following order: Area H‐a, Area H‐b and Area O.ConclusionA high areal ratio of tumor cells and AVD as well as a high expression of stem cells and angiogenic markers were observed in cases of h‐CCC, whereas the areal ratio of fibrous stroma and malignant potential were low. These results suggest that h‐CCC may represent the early stage of CCC.