Simulating calcium influx and free calcium concentrations in yeast.
Simulating calcium influx and free calcium concentrations in yeast.
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DOI：
10.1016/j.ceca.2008.07.005
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发表时间：
2009-02
期刊：
影响因子：
4
通讯作者：
Cunningham, Kyle W.
中科院分区：
文献类型：
--
作者：
Cui, Jiangjun;Kaandorp, Jaap A.;Ositelu, Olufisayo O.;Beaudry, Veronica;Knight, Alicia;Nanfack, Yves F.;Cunningham, Kyle W.
酵母可以在含有非常高的Ca 2+的环境中增殖，主要是由于液泡Ca 2+转运蛋白Pmc 1和Vcx 1的活性。缺乏这些转运蛋白的酵母突变体不能在高Ca 2+环境中生长，但可以通过环境Mg 2+的小幅增加来恢复生长。低细胞外镁离子出现竞争性抑制新的Ca2+内流途径，并减少游离Ca2+在细胞质中的浓度，从发光蛋白水母发光蛋白判断。这些Mg2+敏感的Ca2+内流途径持续yvc1 cch1双突变体。水母发光蛋白的发光痕迹的数学模型的基础上，我们提出在酵母中存在的至少两个钙离子转运蛋白，进行快速反馈抑制响应升高的胞质游离钙离子浓度。最后，我们表明，VCX 1有助于返回细胞内Ca2+向静息水平休克后高胞外Ca2+更有效地比PMC 1和钙调神经磷酸酶，VCX 1和PMC 1的蛋白磷酸酶调节剂，没有检测到的影响，这些因素在其激活的前几分钟内。因此，酵母中Ca2+转运和信号传导的计算建模可以为这个复杂系统的动力学提供重要的见解。
Yeast can proliferate in environments containing very high Ca2+ primarily due to the activity of vacuolar Ca2+ transporters Pmc1 and Vcx1. Yeast mutants lacking these transporters fail to grow in high Ca2+ environments, but growth can be restored by small increases in environmental Mg2+. Low extracellular Mg2+ appeared to competitively inhibit novel Ca2+ influx pathways and to diminish the concentration of free Ca2+ in the cytoplasm, as judged from the luminescence of the photoprotein aequorin. These Mg2+-sensitive Ca2+ influx pathways persisted in yvc1 cch1 double mutants. Based on mathematical models of the aequorin luminescence traces, we propose the existence in yeast of at least two Ca2+ transporters that undergo rapid feedback inhibition in response to elevated cytosolic free Ca2+ concentration. Finally, we show that Vcx1 helps return cytosolic Ca2+ toward resting levels after shock with high extracellular Ca2+ much more effectively than Pmc1 and that calcineurin, a protein phosphatase regulator of Vcx1 and Pmc1, had no detectable effects on these factors within the first few minutes of its activation. Therefore, computational modeling of Ca2+ transport and signaling in yeast can provide important insights into the dynamics of this complex system.
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DOI：
10.1083/jcb.124.3.351
发表时间：
1994-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cunningham KW;Fink GR
通讯作者：
Fink GR
影响因子：
5.3
作者：
Locke, EG;Bonilla, M;Cunningham, KW
通讯作者：
Cunningham, KW
影响因子：
4.8
作者：
MacDiarmid, CW;Gardner, RC
通讯作者：
Gardner, RC
影响因子：
3.3
作者：
Bonilla, M;Cunningham, KW
通讯作者：
Cunningham, KW
影响因子：
3.5
作者：
HALACHMI, D;EILAM, Y
通讯作者：
EILAM, Y