Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line
Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line
复制标题
淫羊藿苷通过 hFOB 1.19 人成骨细胞系中的 BMP-2/Smad4 信号转导途径促进骨形成
DOI：
10.3892/ijmm.2012.1079
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发表时间：
2012-10-01
影响因子：
5.4
通讯作者：
Yang, Min
中科院分区：
文献类型：
--
作者：
Liang, Wenna;Lin, Munan;Yang, Min
淫羊藿苷是中药淫羊藿的主要活性成分，临床上常用于治疗骨质疏松症。然而，淫羊藿苷治疗作用的确切分子机制尚未阐明。本研究的目的是检测淫羊藿苷对细胞活力、碱性磷酸酶（ALP）活性、钙化结节数量的影响，并通过检测骨形态发生蛋白-2（BMP-2）、Smad 4、Cbfa 1/Runx 2、骨保护素（OPG）、hFOB 1.19人成骨细胞系中核因子κ-B配体（RANKL）受体激活剂和OPG/RANKL比值。我们发现淫羊藿苷能显著增加hFOB 1.19细胞的存活率、ALP活性和钙化结节的数量。淫羊藿苷可上调BMP-2、Smad 4、Cbfa 1/Runx 2、OPG、RANKL的表达及OPG/RANKL比值。结果表明淫羊藿苷可通过BMP-2/Smad 4信号转导通路调节hFOB 1.19细胞的成骨过程。
Icariin, the main active compound of the traditional Chinese medicine, Epimedium, is commonly used for the clinical treatment of osteoporosis. However, the precise molecular mechanism of the therapeutic effect of icariin has not been elucidated. The aim of this study was to examine the effect of icariin on cell viability, alkaline phosphatase (ALP) activity, the amount of calcified nodules, and to delineate the molecular mechanism of icariin-enhanced bone formation by investigating the expression of bone morphogenic protein-2 (BMP-2), Smad4, Cbfa1/Runx2, osteoprotegerin (OPG), receptor activator of nuclear factor kappa-B ligand (RANKL) and the OPG/RANKL ratio in the hFOB 1.19 human osteoblastic cell line. We found that icariin significantly increased the cell viability, the activity of ALP and the amount of calcified nodules in the hFOB 1.19 cells. Furthermore, we observed that icariin upregulated the expression of BMP-2, Smad4, Cbfa1/Runx2, OPG, RANKL and the OPG/RANKL ratio. Our results indicate that icariin can modulate the process of bone formation via the BMP-2/Smad4 signal transduction pathway in hFOB 1.19 cells.