Spatiotemporal force and motion in collective cell migration
Spatiotemporal force and motion in collective cell migration
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DOI：
10.1038/s41597-020-0540-5
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发表时间：
2020-06-24
期刊：
影响因子：
9.8
通讯作者：
Notbohm, Jacob
中科院分区：
文献类型：
--
作者：
Saraswathibhatla, Aashrith;Galles, Emmett E.;Notbohm, Jacob
在伤口愈合、形态发生和癌症转移等生物过程中，细胞以集体的方式移动。在集体细胞迁移中，活跃的细胞力如何产生运动仍然不清楚。许多理论模型已经被引入来阐明细胞的主动力和关于集体运动的不同观测之间的关系，例如集体漩涡，振荡和重排。虽然许多模型都具有在细胞层中平衡力的共同特征，但不同模型之间力与运动之间的具体关系不同，这可能导致不同的结论。力和运动的同时实验测量可以帮助测试理论模型的假设和预测。在这里，我们提供了在1毫米圆形岛屿，这是用来计算细胞速度，细胞基板的牵引力，单层应力的细胞的延时图像。额外的数据包括从扰动细胞数密度和肌动球蛋白收缩性的实验。我们希望这个数据集是有用的研究人员感兴趣的集体细胞迁移的力和运动。
Cells move in collective groups in biological processes such as wound healing, morphogenesis, and cancer metastasis. How active cell forces produce the motion in collective cell migration is still unclear. Many theoretical models have been introduced to elucidate the relationship between the cell's active forces and different observations about the collective motion such as collective swirls, oscillations, and rearrangements. Though many models share the common feature of balancing forces in the cell layer, the specific relationships between force and motion vary among the different models, which can lead to different conclusions. Simultaneous experimental measurements of force and motion can aid in testing assumptions and predictions of the theoretical models. Here, we provide time-lapse images of cells in 1 mm circular islands, which are used to compute cell velocities, cell-substrate tractions, and monolayer stresses. Additional data are included from experiments that perturbed cell number density and actomyosin contractility. We expect this data set to be useful to researchers interested in force and motion in collective cell migration.