Field of view advantage of conjugate adaptive optics in microscopy applications.
Field of view advantage of conjugate adaptive optics in microscopy applications.
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DOI：
10.1364/ao.54.003498
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发表时间：
2015-04-10
期刊：
影响因子：
1.9
通讯作者：
Bifano TG
中科院分区：
文献类型：
--
作者：
Mertz J;Paudel H;Bifano TG
样品引起的像差可能会降低光学显微镜的成像性能。消除这些像差影响的一般策略是使用可变形镜 (DM) 进行波前校正。在大多数情况下，DM 与显微镜光瞳共轭放置，称为光瞳自适应光学器件 (AO)。当像差在空间上发生变化时，另一种配置是将 DM 共轭物放置到主要像差源上，称为共轭 AO。我们针对简化情况对两种配置进行了理论和实验比较，其中空间变化的像差是由明确定义的相位屏幕产生的。我们特别关注由此产生的校正视场 (FOV)。研究发现共轭 AO 可提供显着的 FOV 优势。虽然这个结果在天文学界众所周知，但我们的目标是专门为光学显微镜界重新设计它。
The imaging performance of an optical microscope can be degraded by sample-induced aberrations. A general strategy to undo the effect of these aberrations is to apply wavefront correction with a deformable mirror (DM). In most cases the DM is placed conjugate to the microscope pupil, called pupil adaptive optics (AO). When the aberrations are spatially variant an alternative configuration involves placing the DM conjugate to the main source of aberrations, called conjugate AO. We provide a theoretical and experimental comparison of both configurations for the simplified case where spatially variant aberrations are produced by a well defined phase screen. We pay particular attention to the resulting correction field of view (FOV). Conjugate AO is found to provide a significant FOV advantage. While this result is well known in the astronomy community, our goal here is to recast it specifically for the optical microscopy community.