Metalloproteomics: challenges and prospective for clinical research applications.
Metalloproteomics: challenges and prospective for clinical research applications.
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DOI：
10.1586/14789450.2014.876365
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发表时间：
2014-02
影响因子：
3.4
通讯作者：
Finney L
中科院分区：
文献类型：
--
作者：
Fu D;Finney L
金属是重要的辅因子，用于许多关键的细胞过程。例如，锌在胰岛素生物合成中很重要，并且可能在阿尔茨海默病中起作用，但这是如何发生的大部分仍然未知。知道在给定的时间点，哪种金属存在于哪种蛋白质中，将导致对它们如何工作的新见解。正在开发新的工具来研究这一点，具有生物医学应用的潜力。在这份报告中，我们认为这些技术的承诺和局限性。我们提供了可用的技术的简要概述，并讨论了生物医学应用的技术挑战，特别关注必须克服的这些方法的潜力，以实现。
Metals are essential cofactors, utilized in many critical cellular processes. For example, zinc is important in insulin biosynthesis, and may play a role in Alzheimer's disease, but much of how this happens remains unknown. Knowing which metal is in which protein at a given point in time would lead to new insights into how they do their work. New tools are being developed to investigate this, with potential for biomedical applications. In this report, we consider the promise and limitations of these techniques. We provide a brief overview of the techniques available, and a discussion of the technical challenges to biomedical applications, with particular focus on what must be overcome for the potential of these approaches to be achieved.