DISULFIDE BOND ASSIGNMENT IN HUMAN TISSUE INHIBITOR OF METALLOPROTEINASES (TIMP)
DISULFIDE BOND ASSIGNMENT IN HUMAN TISSUE INHIBITOR OF METALLOPROTEINASES (TIMP)
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DOI：
10.1042/bj2680267
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发表时间：
1990-06-01
影响因子：
4.1
通讯作者：
FREEDMAN, RB
中科院分区：
文献类型：
--
作者：
WILLIAMSON, RA;MARSTON, FAO;FREEDMAN, RB
Disulphide bonds in human recombinant tissue inhibitor of metalloproteinases (TIMP) were assigned by resolving proteolytic digests of TIMP on reverse-phase h.p.l.c. and sequencing those peaks judged to contain disulphide bonds by virtue of a change in retention time on reduction. This procedure allowed the direct assignment of Cys-145-Cys-166 and the isolation of two other peptides containing two disulphide bonds each. Further peptide cleavage in conjunction with fast-atom-bombardment m.s. analysis permitted the assignments Cys-1-Cys-70, Cys-3-Cys-90, Cys-13-Cys-124 and Cys-127-Cys-174 from these peptides. The sixth bond Cys-132-Cys-137 was assigned by inference, as the native protein has no detectable free thiol groups.
