MONOCLONAL-ANTIBODIES IDENTIFY THE PROTECTIVE ANTIGENS OF SPOROZOITES OF PLASMODIUM-KNOWLESI
MONOCLONAL-ANTIBODIES IDENTIFY THE PROTECTIVE ANTIGENS OF SPOROZOITES OF PLASMODIUM-KNOWLESI
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DOI：
10.1073/pnas.79.18.5651
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
NUSSENZWEIG, RS
中科院分区：
文献类型：
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作者：
COCHRANE, AH;SANTORO, F;NUSSENZWEIG, RS
用免疫过诺氏疟原虫的小鼠脾细胞与浆细胞瘤细胞融合制备了9种针对诺氏疟原虫孢子子表面抗原的单克隆抗体。用7个单克隆对[35S]蛋氨酸标记的孢子子提取物进行免疫沉淀，鉴定出相同的3种多肽，表观分子量分别为52,000 (Pk52), 50,000 （Pk50）和42,000 （Pk42）。这些抗原也被免疫了诺氏疟原虫的恒河猴血清所识别。脉冲追踪实验表明，高分子量蛋白是Pk42的前体。通过胰蛋白酶处理活孢子，Pk42是表面抗原，而Pk52和Pk50似乎是细胞内抗原。其中3个单克隆抗体也与另一种类人猿疟疾（P. cynomolgi）的孢子子膜抗原反应，1个单克隆抗体与人类疟疾（P. falciparum）的孢子子膜抗原反应。当检测子孢子中和活性时，大多数抗体及其Fab片段，识别Pk52， Pk50和Pk42，消除了寄生虫的感染性。
Nine monoclonal antibodies against surface antigens of sporozoites of the simian malaria parasite P. knowlesi were produced by fusion of the plasmacytoma cells with spleen cells of a mouse immunized with the parasites. Immunoprecipitation of extracts of [35S]methionine-labeled sporozoites with 7 of the monoclonals identified the same 3 polypeptides with apparent MW of 52,000 (Pk52), 50,000 (Pk50) and 42,000 (Pk42). These antigens also were recognized by serum of a rhesus monkey immunized with and protected against P. knowlesi sporozoites. Pulse-chase experiments indicated that the higher MW proteins are precursors of Pk42. As shown by trypsin treatment of viable sporozoites, Pk42 is a surface antigen whereas Pk52 and Pk50 appear to be intracellular. Three of the monoclonal antibodies also reacted with a membrane antigen of sporozoites of another simian malaria, P. cynomolgi, and 1 monoclonal antibody reacted with sporozoites of human malaria, P. falciparum. When assayed for sporozoite neutralizing activity, most of the antibodies and their Fab fragments, which recognize Pk52, Pk50 and Pk42, abolished parasite infectivity.