The Role of miR-107 as a Potential Biomarker and Cellular Factor for Acute Aortic Dissection.
The Role of miR-107 as a Potential Biomarker and Cellular Factor for Acute Aortic Dissection.
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DOI：
10.1089/dna.2020.5506
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发表时间：
2020-09
影响因子：
3.1
通讯作者：
Zanxin Wang;X. Zhuang;Bailang Chen;Dongjie Feng;Gang Li;M. Wei
中科院分区：
文献类型：
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作者：
Zanxin Wang;X. Zhuang;Bailang Chen;Dongjie Feng;Gang Li;M. Wei
急性主动脉夹层（AD）是最严重、死亡率最高的血管疾病之一。它的实际流行率可能被严重低估。我们研究了不同的表达基因，以了解急性 AD 和未患病个体之间的基因谱变化，然后发现急性 AD 的潜在生物标志物和治疗靶点。在我们的研究中，通过对 Gene Expression Omnibus 数据集 GSE52093、GSE98770 和 GSE92427 的生物信息学分析，鉴定了急性 AD 差异表达的 mRNA 和 miRNA。然后，使用综合目标预测和网络分析方法来评估蛋白质-蛋白质相互作用网络并识别差异表达mRNA的基因本体术语。还评估了与急性 AD 相关的差异表达 mRNA-miRNA。最后通过实时定量PCR和体外实验对结果进行了验证。我们发现在急性 AD 组织中整合膜蛋白 2C (ITM2C) 低表达，而 miR-107-5p 高表达。同时，过表达miR-107-5p促进RASMC细胞增殖并抑制细胞凋亡。 miR-107-5p 通过靶向 ITM2C 抑制急性 AD 的进展。
Acute aortic dissection (AD) is one of the most severe and highly mortality vascular disease. Its actual prevalence may be seriously underestimated. We studied different expression genes to understand gene profile change between acute AD and nondiseased individuals, and then discover potential biomarkers and therapeutic targets of acute AD. In our study, acute AD differentially expressed mRNAs and miRNAs were identified through bioinformatics analysis on Gene Expression Omnibus data sets GSE52093, GSE98770, and GSE92427. Then, comprehensive target prediction and network analysis methods were used to evaluate protein-protein interaction networks and to identify Gene Ontology terms for differentially expressed mRNAs. Differentially expressed mRNAs-miRNAs involved in acute AD were assessed as well. Finally, the quantitative real-time PCR and in vitro experiment was used to validate the results. We found Integral Membrane Protein 2C (ITM2C) was low expressed and miR-107-5p was highly expressed in acute AD tissues. Meanwhile, overexpression miR-107-5p promoted the cell proliferation and inhibited the cell apoptosis in RASMC cells. miR-107-5p inhibited the progression of acute AD through targeted ITM2C.