Interleukin-17 mRNA expression in blood and CSF mononuclear cells is augmented in multiple sclerosis
Interleukin-17 mRNA expression in blood and CSF mononuclear cells is augmented in multiple sclerosis
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DOI：
10.1177/135245859900500206
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发表时间：
1999-04-01
影响因子：
5.8
通讯作者：
Link, H
中科院分区：
文献类型：
--
作者：
Matusevicius, D;Kivisäkk, P;Link, H
髓磷脂导向自身免疫被认为在多发性硬化症（MS）的发病机制中起关键作用。白细胞介素-17 （IL-17）是最近发现的一种细胞因子，几乎完全由激活的记忆T细胞产生，它诱导实质细胞和巨噬细胞产生促炎细胞因子和趋化因子。采用人工合成寡核苷酸探针原位杂交技术，对MS患者和对照组血液和脑脊液中表达单核细胞（MNC）的IL-17 mRNA进行检测和计数。与健康人群相比，MS和急性无菌性脑膜脑炎（AM）患者血液中表达MNC的IL-17 mRNA数量较高。与缓解期相比，在临床加重期检查的MS患者中检测到更多表达IL-17 mRNA的血液MNC。与血液相比，MS患者CSF中表达IL-17 mRNA的MNC数量更高。AM患者脑脊液中表达IL-17 mRNA的MNC数量未见增加。因此，我们的研究结果表明，MS中表达MNC的IL-17 mRNA数量增加，CSF中的IL-17 mRNA数量高于血液中的IL-17 mRNA数量，并且在临床加重期间血液中的IL-17 mRNA数量最高。
Myelin-directed autoimmunity is considered to Ploy a key role in the pathogenesis of multiple sclerosis (MS). increased Production of both pro- and anti-inflammatory cytokines is a common finding in MS. Interleukin-17 (IL-17) is a recently described cytokine produced in humans almost exclusively by activated memory T cells, which con induce the production of proinflammatory cytokines and chemokines from parenchymal cells and macrophages. In situ hybridisation with synthetic oligonucleotide probes was adopted to detect and enumerate IL-17 mRNA expressing mononuclear cells (MNC) in blood and cerebrospinal fluid (CSF) from patients with MS and control individuals. Numbers of IL-17 mRNA expressing blood MNC were higher in patients with MS and acute aseptic meningoencephalitis (AM) compared to healthy individuals. Higher numbers of IL-17 mRNA expressing blood MNC were detected in MS patients examined during clinical exacerbation compared to remission. Patients with MS had higher numbers of IL-17 mRNA expressing MNC in CSF compared to blood. This increase in numbers of IL-17 mRNA expressing MNC in CSF was not observed in patients with AM. Our results thus demonstrate increased numbers of IL-17 mRNA expressing MNC in MS with higher numbers in CSF than blood, and with the highest numbers in blood during clinical exacerbations.