Comparison of selected analytical techniques for protein sizing, quantitation and molecular weight determination
Comparison of selected analytical techniques for protein sizing, quantitation and molecular weight determination
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DOI：
10.1016/j.jbbm.2004.01.007
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发表时间：
2004-09-30
影响因子：
--
通讯作者：
Woodward, C
中科院分区：
文献类型：
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作者：
Goetz, H;Kuschel, M;Woodward, C
由于重组DNA技术获得的蛋白质数量越来越多，蛋白质分析技术发展迅速。本文比较了用于蛋白质测定、定量和相对分子质量测定的几种技术：十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法(SDS-PAGE)、芯片实验室或微流控技术(LOAC)、尺寸排除层析(SEC)和质谱仪(MS)。我们从不同的应用领域、分析时间、蛋白质大小和定量性能方面比较了每种技术的优势和局限性。(C)2004爱思唯尔B.V.保留所有权利。
Protein analysis techniques are developing fast due to the growing number of proteins obtained by recombinant DNA techniques. In the present paper we compare selected techniques, which are used for protein sizing, quantitation and molecular weight determination: sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), lab-on-a-chip or microfluidics technology (LoaC), size exclusion chromatography (SEC) and mass spectrometry (MS). We compare advantages and limitations of each technique in respect to different application areas, analysis time, protein sizing and quantitation performance. (C) 2004 Elsevier B.V. All rights reserved.