JAZ repressor proteins are targets of the SCFCO11 complex during jasmonate signalling
JAZ repressor proteins are targets of the SCFCO11 complex during jasmonate signalling
复制标题
DOI：
10.1038/nature05960
复制
发表时间：
2007-08-09
期刊：
影响因子：
64.8
通讯作者：
Browse, John
中科院分区：
文献类型：
--
作者：
Thines, Bryan;Katsir, Leron;Browse, John
茉莉酸盐及其相关信号化合物在植物寄主免疫和发育中起着至关重要的作用，但其信号机制的分子细节尚不清楚。在这里，我们发现茉莉酸zim结构域（JAZ）蛋白家族的成员是茉莉酸信号传导的关键调节因子。JAZ1蛋白可抑制茉莉酸反应基因的转录。茉莉酸盐处理导致JAZ1降解，这种降解依赖于scfco1泛素连接酶和26S蛋白酶体的活性。此外，茉莉异亮氨酸（JA-Ile）偶联物，而不是其他茉莉衍生物，如茉莉酸、12-氧-植物二烯酸或茉莉酸甲酯，在缺乏其他植物蛋白的情况下促进COI1和JAZ1蛋白之间的物理相互作用。我们的研究结果表明，茉莉酸配体促进了scfcoi1泛素连接酶与JAZ1抑制蛋白的结合和随后的降解，并暗示SCFCOI1-JAZ1蛋白复合物是植物激素JA-Ile的感知位点。
Jasmonate and related signalling compounds have a crucial role in both host immunity and development in plants, but the molecular details of the signalling mechanism are poorly understood. Here we identify members of the jasmonate ZIM-domain (JAZ) protein family as key regulators of jasmonate signalling. JAZ1 protein acts to repress transcription of jasmonate-responsive genes. Jasmonate treatment causes JAZ1 degradation and this degradation is dependent on activities of the SCFCOI1 ubiquitin ligase and the 26S proteasome. Furthermore, the jasmonoyl-isoleucine (JA-Ile) conjugate, but not other jasmonate-derivatives such as jasmonate, 12-oxo-phytodienoic acid, or methyl-jasmonate, promotes physical interaction between COI1 and JAZ1 proteins in the absence of other plant proteins. Our results suggest a model in which jasmonate ligands promote the binding of the SCF COI1 ubiquitin ligase to and subsequent degradation of the JAZ1 repressor protein, and implicate the SCFCOI1-JAZ1 protein complex as a site of perception of the plant hormone JA-Ile.