Specificity of ELISA for antibody to beta 2-glycoprotein I in patients with antiphospholipid syndrome.
Specificity of ELISA for antibody to beta 2-glycoprotein I in patients with antiphospholipid syndrome.
复制标题
抗磷脂综合征患者 β2-糖蛋白 I 抗体 ELISA 的特异性。
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
G. Hughes
中科院分区：
文献类型：
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作者：
O. Amengual;T. Atsumi;M. Khamashta;T. Koike;G. Hughes
为探讨抗β 2-GPI抗体在系统性红斑狼疮（SLE）抗磷脂综合征（APS）患者中的临床意义。抗β 2-GPI在120例患者（39例原发性APS，32例APS伴SLE和49例SLE无APS）中通过ELISA使用无心磷脂的辐照平板进行检测。抗心磷脂抗体（aCL）和抗磷脂酰丝氨酸抗体也测量相同的患者使用标准化的测定。抗β 2-GPI滴度与aCL（r = 0.816，P = 0.0001）和抗磷脂酰丝氨酸抗体（r = 0.841，P = 0.0001）密切相关。抗β 2-GPI在APS组中的阳性率为53.5%（38/71），而在无APS的SLE组中仅为4.1%（2/49）。在后一组中，24.5%（12/49）的患者具有aCL的阳性滴度。抗β 2-GPI检测对APS的特异性高于APS中aCL的特异性（分别为96%和75%，卡方2 = 6.75，P = 0.00094）。本研究结果提示，抗β 2-GPI抗体检测可提高APS的特异性。
The clinical significance of anti-beta 2 glycoprotein I (beta 2-GPI) antibodies was evaluated in patients with antiphospholipid syndrome (APS), primary and secondary to systemic lupus erythematosus (SLE). Anti-beta 2-GPI were tested in 120 patients (39 primary APS, 32 APS with SLE and 49 SLE without APS) by ELISA utilizing irradiated plates in the absence of cardiolipin. Anticardiolipin antibodies (aCL) and antiphosphatidylserine antibodies were also measured in the same patients using standardized assays. Anti-beta 2-GPI titres correlated strongly to those of aCL (r = 0.816, P = 0.0001), and to those of antiphosphatidylserine antibodies (r = 0.841, P = 0.0001). Anti-beta 2-GPI were detected in 53.5% of APS patients (38/71), but only in 4.1% of SLE patients without APS (2/49). In the latter group, 24.5% (12/49) of patients had a positive titre of aCL. The anti-beta 2-GPI assay showed higher specificity for APS than the aCL in APS (96 vs 75%, respectively, chi 2 = 6.75, P = 0.00094). Our findings suggest that the assay of anti-beta 2-GPI may improve the specificity for APS.