Extracellular adenosine levels and cellular energy metabolism in ischemically preconditioned rat heart
Extracellular adenosine levels and cellular energy metabolism in ischemically preconditioned rat heart
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DOI：
10.1016/s0008-6363(98)00123-0
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发表时间：
1998-10-01
影响因子：
10.8
通讯作者：
Headrick, JP
中科院分区：
文献类型：
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作者：
Harrison, GJ;Willis, RJ;Headrick, JP
目的：微透析和P-31-NMR光谱被用来测试相反的假设，缺血预处理抑制腺嘌呤核苷酸降解和嘌呤流出，或预处理激活心血管腺苷形成，以提供增强的心脏保护。方法：从Langendorff灌注的大鼠心脏获得31 P-NMR谱和匹配的组织间液（ISF）或静脉流出物样品。对照组（n=9）进行30分钟的全脑常温缺血和30分钟的再灌注。预处理的心脏（n=6）进行5分钟的缺血发作和10分钟的再灌注前30分钟的缺血和30分钟的再灌注。还评估了重复缺血-再灌注（3 × 5分钟缺血发作）对腺苷水平和能量代谢的影响（n=8）。结果：预处理可改善缺血后心率恢复和左室发展压（71+/-5vs43 +/-8%，P
Objective: Microdialysis and P-31-NMR spectroscopy were used to test opposing hypotheses that ischemic preconditioning inhibits adenine nucleotide degradation and purine efflux, or that preconditioning activates cardiovascular adenosine formation to provide enhanced cardioprotection. Methods: 31P-NMR spectra and matching interstitial fluid (ISF) or venous effluent samples were obtained from Langendorff perfused rat hearts. Control hearts (n=9) underwent 30 min of global normothermic ischemia and 30 min reperfusion. Preconditioned hearts (n=6) were subjected to a 5 min ischemic episode and 10 min reflow prior to 30 min ischemia and 30 min reperfusion. Effects of repetitive ischemia-reperfusion (3x5 min ischemic episodes) on adenosine levels and energy metabolism were also assessed (n=8). Results: Preconditioning improved post-ischemic recovery of heart rate x left ventricular developed pressure (71+/-5 vs 43+/-8%, P