Attenuated phosphorylation of heat shock protein 27 correlates with tumor progression in patients with hepatocellular carcinoma.
Attenuated phosphorylation of heat shock protein 27 correlates with tumor progression in patients with hepatocellular carcinoma.
复制标题
热休克蛋白 27 的磷酸化减弱与肝细胞癌患者的肿瘤进展相关。
DOI：
10.1016/j.bbrc.2005.08.273
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发表时间：
2005
影响因子：
3.1
通讯作者：
O. Kozawa
中科院分区：
文献类型：
--
作者：
E. Yasuda;T. Kumada;S. Takai;A. Ishisaki;Takahiro Noda;R. Matsushima;N. Yoshimi;Kanefusa Kato;H. Toyoda;Y. Kaneoka;A. Yamaguchi;O. Kozawa
热休克蛋白27（HSP 27）在人肝细胞癌（HCC）中高表达。我们研究了48例肝癌切除标本中总HSP 27和丝氨酸磷酸化（Ser-15，Ser-78和Ser-82）HSP 27水平与临床和病理特征的相关性。Western blot分析总HSP 27和丝氨酸磷酸化HSP 27的水平。还对一些样品进行了HSP 27表达的免疫组织化学分析。48例HCC组织中均检测到HSP 27磷酸化。磷酸化HSP 27水平与肿瘤大小、微血管浸润和TNM分期呈负相关。相反，只有微血管浸润与总HSP 27水平呈负相关。通过免疫组化也观察到进展性HCC中磷酸化HSP 27的减少。磷酸化HSP 27水平随着HCC进展而逐渐降低。我们的研究结果表明，磷酸化HSP 27可能有抑制作用，在人类肝癌的进展。
Heat shock protein 27 (HSP27) is expressed at high levels in human hepatocellular carcinoma (HCC). We examined correlations of total HSP27 and serine phosphorylated (Ser-15, Ser-78, and Ser-82) HSP27 levels in HCC tissues with clinical and pathologic characteristics in 48 resected HCC specimens. The levels of total and Ser-phosphorylated HSP27 were evaluated by Western blot analysis. Immunohistochemical analysis of HSP27 expression was also performed on some samples. Phosphorylation of HSP27 was detected in all 48 HCC tissues. Levels of phosphorylated HSP27 were correlated inversely with tumor size, microvascular invasion of HCC, and tumor stage by TNM classification. In contrast, only microvascular invasion showed an inverse correlation with total HSP27 levels. The decrease in phosphorylated HSP27 in progressed HCC was also observed by immunohistochemistry. Levels of phosphorylated HSP27 gradually decreased in parallel with HCC progression. Our findings suggest that phosphorylated HSP27 may have a suppressive role in progression of human HCC.