The brassinosteroid‐responsive xyloglucan endotransglucosylase/hydrolase 19 ( XTH19 ) and XTH23 genes are involved in lateral root development under salt stress in Arabidopsis
The brassinosteroid‐responsive xyloglucan endotransglucosylase/hydrolase 19 ( XTH19 ) and XTH23 genes are involved in lateral root development under salt stress in Arabidopsis
复制标题
油菜素类固醇响应性木葡聚糖内转葡糖基酶/水解酶 19 ( XTH19 ) 和 XTH23 基因参与拟南芥盐胁迫下的侧根发育
DOI：
10.1111/tpj.14905
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发表时间：
2020
期刊：
影响因子：
7.2
通讯作者：
Weiming Cai
中科院分区：
文献类型：
--
作者：
Peipei Xu;Shan Fang;Haiying Chen;Weiming Cai
侧根是拟南芥根系构型的主要组成部分。LR发育的可塑性在提高植物对外界环境的反应中具有重要作用。先前的研究已经揭示了许多控制植物生长以响应环境刺激的遗传途径。在此，我们发现木葡聚糖内切转葡糖基酶19（XTH 19）和XTH 23基因参与盐胁迫下LR的发育。与野生型相比，单突变体xth23的LRs密度降低，对盐的敏感性增强，而双突变体xth19 xth23的LRs起始和盐敏感性均呈加性下调。相反，组成型过表达XTH 19或XTH 23导致LR密度增加。此外，XTH 19和XTH 23通过油菜素类固醇信号途径关键转录因子BES 1被盐诱导。此外，我们还发现35S：：BES 1基因增强了XTH 19和XTH 23的耐盐性，而且xth 19和XTH 23的表型与野生型部分互补，体内和体外实验表明BES 1基因直接作用于XTH 19和XTH 23的上游，控制它们的表达。总体而言，我们的研究结果表明，XTH 19和XTH 23通过BES 1依赖性途径参与LR的发育，并有助于LR对盐的适应。
Lateral roots (LRs) are the main component of the root system architecture in Arabidopsis. The plasticity of LR development has an important role in improving plant survival in response to the external environment. Previous studies have revealed a number of genetic pathways that control plant growth in response to environmental stimuli. Here, we find that the xyloglucan endotransglucosylase 19 (XTH19) andXTH23genes are involved in LR development under salt stress. The density of LRs was decreased in thexth23single mutant, which was also more sensitive to salt than the wild type, and thexth19xth23double mutant exhibited additive downregulated LR initiation and salt sensitivity compared with the single mutant. On the contrary, constitutive overexpression ofXTH19orXTH23caused increased LR densities. Furthermore,XTH19andXTH23were induced by salt via the key brassinosteroid signaling pathway transcription factorBES1. In addition, we found that 35S::BES1increased salt tolerance and the phenotype ofxth19xth23& 35S::BES1was partially complementary to the wild‐type level.In vivoandin vitroassays demonstrated thatBES1acts directly upstream ofXTH19andXTH23to control their expression. Overall, our results revealed thatXTH19andXTH23are involved in LR development via theBES1‐dependent pathway, and contribute to LR adaptation to salt.