Increased naive CD4+ and B lymphocyte subsets are associated with body mass loss and drive relative lymphocytosis in anorexia nervosa patients
Increased naive CD4+ and B lymphocyte subsets are associated with body mass loss and drive relative lymphocytosis in anorexia nervosa patients
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DOI：
10.1016/j.nutres.2017.02.006
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发表时间：
2017-03-01
影响因子：
4.5
通讯作者：
Nova, Esther
中科院分区：
文献类型：
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作者：
Elegido, Ana;Graell, Montserrat;Nova, Esther
神经性厌食症（AN）是一种非典型形式的营养不良，免疫系统发生特殊变化。我们假设不同的淋巴细胞亚群受到AN营养不良的不同影响，因此，我们的目的是研究体重减轻对AN患者淋巴细胞亚群变异性的影响。一组66例青少年女性患者，年龄12-17岁，在入院时因AN或未分类的进食或进食障碍首次发作而转诊（46例AN限制亚型，11例AN狂欢/清除亚型和9例未分类的进食或进食障碍）。90名健康青少年作为对照组。流式细胞术分析白色血细胞和淋巴细胞亚群。与体重指数（BMI）z评分的关系在线性模型中进行了评估，并根据诊断亚型和年龄进行了调整。AN患者的白细胞数量低于对照组，AN限制亚型中观察到相对淋巴细胞增多。与对照组相比，进食障碍患者的CD 8（+）、NK和记忆CD 8（+）计数较低。两组间CD 4（+）计数或幼稚和记忆CD 4（+）亚群无差异。淋巴细胞百分比和BMI z评分之间以及B细胞计数、初始CD 4（+）百分比和计数与BMI z评分之间存在负相关性。总之，与体重减轻相关的初始CD 4（+）和B淋巴细胞亚群的增加驱动了AN患者中观察到的相对淋巴细胞增多，这反映了维持适应性免疫应答的适应性机制。(C)2017爱思唯尔公司All rights reserved.
Anorexia nervosa (AN) is an atypical form of malnutrition with peculiar changes in the immune system. We hypothesized that different lymphocyte subsets are differentially affected by malnutrition in AN, and thus, our aim was to investigate the influence of body mass loss on the variability of lymphocyte subsets in AN patients. A group of 66 adolescent female patients, aged 12-17 years, referred for their first episode of either AN or feeding or eating disorders not elsewhere classified were studied upon admission (46 AN-restricting subtype, 11 AN-binge/purging subtype, and 9 feeding or eating disorders not elsewhere classified). Ninety healthy adolescents served as controls. White blood cells and lymphocyte subsets were analyzed by flow cytometry. Relationships with the body mass index (BMI) z score were assessed in linear models adjusted by diagnostic subtype and age. Leukocyte numbers were lower in AN patients than in controls, and relative lymphocytosis was observed in AN-restricting subtype. Lower CD8(+), NK, and memory CD8(+) counts were found in eating disorder patients compared with controls. No differences were found for CD4(+) counts or naive and memory CD4(+) subsets between the groups. Negative associations between lymphocyte percentage and the BMI z score, as well as between the B cell counts, naive CD4(+) percentage and counts, and the BMI z score, were found. In conclusion, increased naive CD4(+) and B lymphocyte subsets associated with body mass loss drive the relative lymphocytosis observed in AN patients, which reflects an adaptive mechanism to preserve the adaptive immune response. (C) 2017 Elsevier Inc. All rights reserved.