EXPRESSION OF THE HOMEO BOX CONTAINING GENE EN-2 DELINEATES A SPECIFIC REGION OF THE DEVELOPING MOUSE-BRAIN
EXPRESSION OF THE HOMEO BOX CONTAINING GENE EN-2 DELINEATES A SPECIFIC REGION OF THE DEVELOPING MOUSE-BRAIN
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DOI：
10.1101/gad.2.3.361
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发表时间：
1988-03-01
影响因子：
10.5
通讯作者：
JOYNER, AL
中科院分区：
文献类型：
--
作者：
DAVIS, CA;NOBLETOPHAM, SE;JOYNER, AL
我们利用原位杂交研究了小鼠胚胎发生过程中含有同源盒的基因 En-2 的表达模式。转录本首先在 8 天、5 个体节胚胎的神经褶皱中检测到，并且在整个发育过程中持续表达到成年。杂交仅发生在中枢神经系统（CNS）中，并且仅限于神经管的一条带以及后来从神经管发展而来的部分结构；小脑、脑桥、导水管周围灰质和丘。 CNS 生发区的表达在杂交带内是一致的。然而，在发育后期，一旦细胞迁移出生发区，杂交程度就会减少，空间复杂性就会增加。在成人中，En-2 的表达似乎仅限于特定的神经元组。 En-2 在明显同质的组织内的早期局部表达与 En-2 在定义发育中的大脑内的空间域中发挥作用的假设是一致的。
We have examined the pattern of expression of the homeo box-containing gene En-2 during mouse embryogenesis using in situ hybridization. Transcripts were first detected in the neural folds of 8.0-day, 5-somite embryos, and expression continued throughout development into adulthood. Hybridization occurred only in the central nervous system (CNS) and was limited to one band of the neural tube and to parts of those structures the later developed from it; the cerebellum, pons, periaqueductal gray, and colliculi. Expression in the germinal zone of the CNS was uniform within the hybridizing band. However, later in development, once cells had migrated out of the germinal zone, there was a reduction in the extent of hybridization and an increase in its spatial complexity. In the adult, expression of En-2 appeared to be limited to specific groups of neurons. The early, localized expression of En-2 within an apparently homogeneous tissue is consistent with the hypothesis that En-2 plays a role in defining a spatial domain within the developing brain.