Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity.
Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity.
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肌酸阻止了肌源性，氧化压力C2C12细胞的线粒体对线粒体的结构和功能损害，并恢复其分化能力。
DOI：
10.1155/2016/5152029
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发表时间：
2016
影响因子：
--
通讯作者：
Sestili P
中科院分区：
文献类型：
--
作者：
Barbieri E;Guescini M;Calcabrini C;Vallorani L;Diaz AR;Fimognari C;Canonico B;Luchetti F;Papa S;Battistelli M;Falcieri E;Romanello V;Sandri M;Stocchi V;Ciacci C;Sestili P
肌酸（Cr）是一种营养补充剂，促进许多健康益处。事实上，铬已被证明是有益的疾病引起的肌肉萎缩，改善康复，并提供温和的抗氧化活性。有益的效果可能来自多效性相互作用。与此概念雅阁，我们以前证明了多种多效性效应，包括线粒体损伤的保护，解释了Cr阻止C2 C12成肌细胞中氧化应激引起的分化停滞的能力。鉴于线粒体在支持肌生成过程中的重要性，本文进一步探讨了Cr对氧化应激条件下分化的C2 C12细胞中这些细胞器的结构、功能和网络的保护作用;还研究了对能量传感器AMPK、参与线粒体生物合成的PGC-1α及其下游效应子Tfam的影响。我们的研究结果表明，线粒体的损伤是至关重要的氧化应激引起的分化不平衡和铬-预防这些损伤总是与正常的生肌能力的恢复。我们还发现Cr激活AMPK并诱导PGC-1α表达上调，这两个事件可能有助于保护线粒体质量和功能。
Creatine (Cr) is a nutritional supplement promoting a number of health benefits. Indeed Cr has been shown to be beneficial in disease-induced muscle atrophy, improve rehabilitation, and afford mild antioxidant activity. The beneficial effects are likely to derive from pleiotropic interactions. In accord with this notion, we previously demonstrated that multiple pleiotropic effects, including preservation of mitochondrial damage, account for the capacity of Cr to prevent the differentiation arrest caused by oxidative stress in C2C12 myoblasts. Given the importance of mitochondria in supporting the myogenic process, here we further explored the protective effects of Cr on the structure, function, and networking of these organelles in C2C12 cells differentiating under oxidative stressing conditions; the effects on the energy sensor AMPK, on PGC-1α, which is involved in mitochondrial biogenesis and its downstream effector Tfam were also investigated. Our results indicate that damage to mitochondria is crucial in the differentiation imbalance caused by oxidative stress and that the Cr-prevention of these injuries is invariably associated with the recovery of the normal myogenic capacity. We also found that Cr activates AMPK and induces an upregulation of PGC-1α expression, two events which are likely to contribute to the protection of mitochondrial quality and function.
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影响因子：
5.5
作者：
Ceddia, RB;Sweeney, G
通讯作者：
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影响因子：
11.4
作者：
Biswas, G;Adebanjo, OA;Avadhani, NG
通讯作者：
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DOI：
10.1073/pnas.0705070104
发表时间：
2007-07-17
影响因子：
11.1
作者：
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作者：
Ekstrand, MI;Falkenberg, M;Larsson, NG
通讯作者：
Larsson, NG
DOI：
10.1073/pnas.69.8.2250
发表时间：
1972-01-01
影响因子：
11.1
作者：
INGWALL, JS;STOCKDAL.FE;MORALES, MF
通讯作者：
MORALES, MF