N-methyl-D-aspartate receptor and apoptosis in Alzheimer's disease and multiinfarct dementia
N-methyl-D-aspartate receptor and apoptosis in Alzheimer's disease and multiinfarct dementia
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DOI：
10.1002/jnr.20558
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发表时间：
2005-07-15
影响因子：
4.2
通讯作者：
Yew, DT
中科院分区：
文献类型：
--
作者：
Fang, MR;Li, JC;Yew, DT
本研究通过免疫组织化学方法检测阿尔茨海默病和多发性梗死性痴呆患者额叶皮质中N-甲基-D-天冬氨酸（NMDA）受体阳性细胞的数量以及NMDA受体与凋亡标志物（如TUNEL或活化的caspase-3）之间的共定位程度，并与那些死于正常衰老的受试者的结果进行比较。我们发现，在阿尔茨海默病患者的额叶皮层，特别是皮层的深层，NMDA受体阳性细胞和TUNEL标记细胞的数量增加。然而，只有约10%的细胞表现出共定位的NMDA受体与凋亡标记物的研究，这表明NMDA介导的兴奋性毒性并没有发挥重要作用，在阿尔茨海默病或多发性梗死性痴呆的神经元凋亡。(c)2005 Wiley-Liss，Inc.
This study investigates the role of excitotoxicity in Alzheimer's disease and in multiinfarct dementia by examining, via immunohistochemical methods, the number of cells that are positive for N-methyl-D-aspartate (NMDA) receptor and the degree of colocalization between NMDA receptor and apoptosis markers such as TUNEL or activated caspase-3 in the frontal cortex of individuals with these two conditions, comparing the results with those from subjects who died of normal aging. We showed an increased number of NMDA receptor-positive cells and an increased number of TUNEL-labeled cells in the frontal cortex of subjects with Alzheimer's disease, especially in the deeper layers of the cortex. However, only about 10% of cells showed colocalization of NMDA receptor with the apoptosis markers studied, suggesting that NMDA-mediated excitotoxicity does not play a major role in neuronal apoptosis in Alzheimer's disease or in multiinfarct dementia. (c) 2005 Wiley-Liss, Inc.