CLONAL ORIGINS OF CELLS IN THE PIGMENTED RETINA OF THE ZEBRAFISH EYE
CLONAL ORIGINS OF CELLS IN THE PIGMENTED RETINA OF THE ZEBRAFISH EYE
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DOI：
10.1016/s0012-1606(89)80038-7
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发表时间：
1989-01-01
影响因子：
2.7
通讯作者：
GRUNWALD, DJ
中科院分区：
文献类型：
--
作者：
STREISINGER, G;COALE, F;GRUNWALD, DJ
斑马鱼（Brachydanio rerio）色素视网膜发育的克隆基础已被应用于马赛克分析。将gol1基因座隐性突变杂合的斑马鱼胚胎暴露于。γ。在不同的发育阶段进行辐照，以在眼睛中产生由野生型色素细胞和无色素（金色）细胞克隆组成的马赛克个体。测量了单个胚胎细胞对色素视网膜的贡献，并确定了胚胎中为该组织贡献后代的细胞总数。直到32个细胞阶段，几乎每个卵裂球都有一些后代参与斑马鱼色素视网膜的形成。在随后的细胞分裂中，直到几千个细胞阶段，祖先细胞的数量是恒定的：大约有40个细胞存在，这些细胞将在色素视网膜中产生后代。对色素视网膜克隆大小的分析表明，这种组织的细胞不是通过起源于早期胚胎的一系列严格的细胞分裂产生的。研究发现，每个卵裂期细胞都对色素视网膜有贡献，但这些细胞的贡献是高度可变的，这与不同卵裂球的克隆后代通常在色素视网膜形成之前广泛混合的解释一致。
Mosaic analysis has been used to study the clonal basis of the development of the pigmented retina of the zebrafish, Brachydanio rerio. Zebrafish embryos heterozygous for a recessive mutation at the gol-1 locus were exposed to .gamma.-irradiation at various developmental stages to create mosaic individuals consisting of wild-type pigmented cells and a clone of pigmentless (golden) cells in the eye. The contribution of individual embryonic cells to the pigmented retina was measured and the total number of cells in the embryo that contributed descendants to this tissue was determined. Until the 32-cell stage, almost every blastomere has some descendants that participate in the formation of the pigmented retina of the zebrafish. During subsequent cells divisions, up to several thousand-cell stage, the number of ancestral cell is constant: approximately 40 cells are present that will give rise to progeny in the pigmented retina. Analysis of the size of clones in the pigmented retina indicates that the cells of this tissue do not arise through a rigid series of cell divisions originating in the early embryo. The findings that each cleavage stage cell contributes to the pigmented retina and yet the contribution of such cells is highly variable are consistent with the interpretation that clonal descendants of different blastomeres normally intermix extensively prior to formation of the pigmented retina.