Nicotinic antagonists enhance process outgrowth by rat retinal ganglion cells in culture.
Nicotinic antagonists enhance process outgrowth by rat retinal ganglion cells in culture.
复制标题
烟碱拮抗剂可增强培养中的大鼠视网膜神经节细胞的生长过程。
DOI：
10.1126/science.3344435
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Aizenman,E
中科院分区：
文献类型：
--
作者：
Lipton,SA;Frosch,MP;Phillips,MD;Tauck,DL;Aizenman,E
在培养的哺乳动物视网膜神经节细胞神经元上发现了功能性烟碱胆碱能受体。神经递质乙酰胆碱（ACh）可以检测到许多这些视网膜文化的介质中，释放后，大概从胆碱乙酰转移酶阳性无长突细胞。内源性或应用ACh对神经节细胞的突触后作用可以被特异性烟碱拮抗剂阻断。这里显示，在产生这种药理学阻断的24小时内，视网膜神经节细胞开始发芽或再生神经元突起。因此，烟碱拮抗剂的生长促进作用可能是由于消除了培养基中存在的ACh的紧张水平对生长的抑制。由于在完整的视网膜中存在乙酰胆碱的自发泄漏，烟碱胆碱能药物对培养中的过程生长的影响可能反映了由乙酰胆碱在体内施加的视网膜神经节细胞过程的生长或再生的正常控制机制。
Functional nicotinic cholinergic receptors are found on mammalian retinal ganglion cell neurons in culture. The neurotransmitter acetylcholine (ACh) can be detected in the medium of many of these retinal cultures, after release presumably from the choline acetyltransferase-positive amacrine cells. The postsynaptic effect of endogenous or applied ACh on the ganglion cells can be blocked with specific nicotinic antagonists. Here it is shown that within 24 hours of producing such a pharmacologic blockade, the retinal ganglion cells begin to sprout or regenerate neuronal processes. Thus, the growth-enhancing effect of nicotinic antagonists may be due to the removal of inhibition to growth by tonic levels of ACh present in the culture medium. Since there is a spontaneous leak of ACh in the intact retina, the effects of nicotinic cholinergic drugs on process outgrowth in culture may reflect a normal control mechanism for growth or regeneration of retinal ganglion cell processes that is exerted by ACh in vivo.