Relating alternative splicing to proteome complexity and genome evolution.
Relating alternative splicing to proteome complexity and genome evolution.
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DOI：
10.1007/978-0-387-77374-2_3
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发表时间：
2007
影响因子：
--
通讯作者：
Yi Xing;Christopher J. Lee
中科院分区：
文献类型：
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作者：
Yi Xing;Christopher J. Lee
在基因组学出现之前，选择性剪接的研究主要集中在单个基因和外显子中选择性剪接的功能和机制。自20世纪90年代末以来，这种情况发生了巨大变化。高通量基因组学技术，如EST测序和设计用于检测剪接变化的微阵列，导致了从人类到拟南芥的广泛物种中的全基因组发现和可变剪接的定量。1，2人类基因组中AS频率的共识估计从20世纪90年代中期的不到5%增长到现在的60-74%。3.可变剪接的序列和微阵列数据的快速增长使得研究可变剪接对蛋白质功能和基因组进化的全球影响成为可能。在这一章中，我们回顾了最近的研究选择性剪接的影响蛋白质组的复杂性和它在基因组进化中的作用。
Prior to genomics, studies of alternative splicing primarily focused on the function and mechanism of alternative splicing in individual genes and exons. This has changed dramatically since the late 1990s. High-throughput genomics technologies, such as EST sequencing and microarrays designed to detect changes in splicing, led to genome-wide discoveries and quantification of alternative splicing in a wide range of species from human to Arabidopsis. 1, 2 Consensus estimates of AS frequency in the human genome grew from less than 5% in mid-1990s to as high as 60–74% now. 3 The rapid growth in sequence and microarray data for alternative splicing has made it possible to look into the global impact of alternative splicing on protein function and evolution of genomes. In this chapter, we review recent research on alternative splicing’s impact on proteomic complexity and its role in genome evolution.