A defect in atToc159 of Arabidopsis thaliana causes severe defects in leaf development
A defect in atToc159 of Arabidopsis thaliana causes severe defects in leaf development
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DOI：
10.1266/ggs.79.207
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发表时间：
2004-08-01
影响因子：
1.1
通讯作者：
Machida, Y
中科院分区：
文献类型：
--
作者：
Asano, T;Yoshioka, Y;Machida, Y
质体蛋白导入2（Plastid protein import 2，ppi2）是拟南芥的一个突变体，它与豌豆叶绿体外被膜上的一个转位子（translocon）Toc159同源。Toc159被认为是光合蛋白进入叶绿体的关键。为了研究蛋白质输入对植物发育的影响，我们对ppi2植物发育中的叶片和茎尖分生组织（SAM）的形态进行了观察。我们的组织学分析表明，PPI2叶片的发育受到严重影响，而SAM结构正常。在叶片发育的后期，叶片中的脱落细胞变得明显，导致与野生型相比，成熟叶片的叶肉细胞较少，细胞间隙较多。ppi2成熟叶片的栅栏和海绵组织难以区分。叶绿体DNA的复制也被认为是ppi2受损。我们的结果表明，蛋白质输入叶绿体是重要的叶片的正常发育。
Plastid protein import 2 (ppi2), a mutant of Arabidopsis thaliana, lacks a homologue of a component of the translocon at the outer envelope membrane of chloroplasts (Toc), designated Toc159 of the pea. Toc159 is thought to be essential for the import of photosynthetic proteins into chloroplasts. In order to investigate the effect of protein import on the plant development, we examined the morphologies of the developing leaves and the shoot apical meristems (SAM) in the ppi2 plants. Our histological analysis revealed that the development of leaves is severely affected in ppi2, while the structure of SAM is normal. Abnormalities in leaves became obvious in the later stages of leaf development, resulting in the generation of mature leaves with fewer mesophyll cells and more intercellular spaces as compared with the wild type. Palisade and spongy tissues of the mature leaves were indistinguishable in ppi2. Replication of chloroplast DNA was also suggested to be impaired in ppi2. Our results suggest that protein import into chloroplasts is important for the normal development of leaves.