Zinc-containing Mohs' paste affects blood flow and angiogenesis suppression
Zinc-containing Mohs' paste affects blood flow and angiogenesis suppression
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DOI：
10.1007/s40199-021-00409-5
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发表时间：
2021-08-21
影响因子：
3.6
通讯作者：
Izumo, Nobuo
中科院分区：
文献类型：
--
作者：
Nagashima, Daichi;Furukawa, Megumi;Izumo, Nobuo
目的莫氏膏是一种由氯化锌和氧化锌淀粉组成的膏体，用于临床浅表恶性肿瘤的止血。方法雄性C57BL/6JJmsSlc小鼠单侧后肢手术后分别给予25%、50%和75%的莫氏膏单次剂量，对照组给予黏度调节剂甘油（0%）。用激光多普勒灌注成像系统测量后肢血流量水平（n = 6）。分别采用电感耦合等离子体质谱法（ICP-MS）和免疫印迹法（western blotting）分析肌肉内锌含量和血管内皮生长因子- a （VEGF-A）表达（n = 5或3）。结果50%组8 h后血流量显著降低，25%、50%组24 h后血流量显著降低，50%、75%组8 h后肌内锌含量显著升高。Western blotting结果显示，25%、50%组8 h后VEGF-A水平显著升高。我们预测了莫氏膏的药理作用，发现超过50%的莫氏膏在应用超过8小时后对血流和血管生成的抑制至关重要。结论血流量抑制的机制与VEGF-A水平无关，可能抑制未来的血管生成。我们的发现支持了先前的研究，莫氏膏有望诱导止血和抑制血管生成。这是一种优秀的软膏，通过抑制血流而促进止血，而不考虑血管生成，并且可能适用于临床需要止血的情况。
Purpose Mohs' paste, which is composed of zinc chloride and zinc oxide starch, is used for hemostasis of superficial malignancy in the clinical setting. We investigated the concentration of intramuscular zinc in mice after Mohs' paste application and evaluated its relationship with angiogenesis from the perspective of blood flow levels within 24 h.Methods Male C57BL/6JJmsSlc mice were administered single dose of Mohs' paste at 25%, 50%, and 75% after unilateral hind limb surgery, and glycerin, a viscosity modifier, was administered to the control group (0%). Hind limb blood flow levels were measured with a laser Doppler perfusion imaging system (n = 6). The amounts of intramuscular zinc and vascular endothelial growth factor-A (VEGF-A) expression were analyzed using inductively coupled plasma mass spectrometry (ICP-MS) and western blotting, respectively (n = 5 or 3).Results Blood flow levels were significantly decreased in the 50% group after 8 h, and significantly decreased in the 25% and 50% groups after 24 h. Intramuscular zinc was significantly increased in the 50% and 75% groups after 8 h. Western blotting showed that VEGF-A levels were significantly increased in the 25% and 50% groups after 8 h. Based on analytical experiments and biological investigation, we predicated the pharmacological effect of Mohs' paste and found over 50% of it is critical in the blood flow and angiogenesis suppression after more than 8 h of its application.Conclusions The results suggest that the mechanism of blood flow suppression is independent of VEGF-A levels and might suppress future angiogenesis. Our findings support that of previous studies, in which Mohs' paste was expected to induce hemostasis and suppress angiogenesis. It is an excellent ointment that facilitates hemostasis by suppressing blood flow regardless of angiogenesis, and may be apt for situations where hemostasis is required in the clinical setting.