Protein Microarrays as a Tool to Analyze Antibody Responses to Variant Surface Antigens Expressed on the Surface of Plasmodium falciparum-Infected Erythrocytes.
Protein Microarrays as a Tool to Analyze Antibody Responses to Variant Surface Antigens Expressed on the Surface of Plasmodium falciparum-Infected Erythrocytes.
复制标题
蛋白质微阵列作为分析抗体对恶性疟原虫感染的红细胞表面表达的变异表面抗原的反应的工具。
DOI：
10.1007/978-1-0716-2189-9_25
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Travassos,MarkA
中科院分区：
文献类型：
--
作者：
Zhou,AlbertE;Jain,Aarti;Nakajima,Rie;Shrestha,Biraj;Stucke,EmilyM;Joshi,Sudhaunshu;Strauss,KathyA;Hedde,PerN;Berry,AndreaA;Felgner,PhilipL;Travassos,MarkA
酶联免疫吸附测定（ELISA）仍然是测量抗体的金标准，但耗时且使用大量珍贵样品和试剂。蛋白质微阵列是一个有吸引力的替代方案，特别是对于那些集中在大型基因家族的研究，如那些编码变异的表面抗原在疟疾寄生虫恶性疟原虫。这种微阵列代表了一个理想的高通量平台，可以同时研究数百种疟原虫变体表面抗原的抗体反应，为疟疾天然免疫的发展提供重要的见解。我们描述了使用aP进行测定的基本背景和方法。恶性疟原虫微阵列填充变异表面抗原。这允许用户定义血清学谱并鉴定代表疫苗或治疗开发的有希望靶点的血清显性抗原。
Enzyme-linked immunosorbent assays (ELISAs) remain the gold standard for measuring antibodies, but are time-consuming and use significant amounts of precious sample and reagents. Protein microarrays represent an appealing alternative, particularly for studies focused on large gene families such as those encoding variant surface antigens in the malaria parasitePlasmodium falciparum. Such microarrays represent an ideal high-throughput platform to study antibody responses to hundreds of malaria parasite variant surface antigens at once, providing critical insights into the development of natural immunity to malaria. We describe the essential background and approach to run an assay using aP. falciparummicroarray populated with variant surface antigens. This allows the user to define serologic profiles and identify serodominant antigens that represent promising targets for vaccine or therapeutic development.