Comparative Transcriptome Analysis Revealed the Freezing Tolerance Signaling Events in Winter Rapeseed (Brassica rapa L.).
Comparative Transcriptome Analysis Revealed the Freezing Tolerance Signaling Events in Winter Rapeseed (Brassica rapa L.).
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比较转录组分析揭示了冬油菜 (Brassica rapa L.) 的耐冻性信号事件
DOI：
10.3389/fgene.2022.871825
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发表时间：
2022
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
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作者：
冬用油菜(Brassica Rapa L.)是中国西北地区重要的油料作物。冰冻胁迫严重限制了其产量和地理分布，气候变化导致的极端冰冻事件频繁发生，增加了冬季冻害的可能性。然而，白菜对冰冻胁迫的反应的潜在机制仍然不清楚。本研究以油菜基因组(v3.0)为参照，对龙优6号和天优2号(耐冷性较强和较弱)在不同冻害条件下的转录水平进行了比较分析。在冻害前后，分别鉴定了5982个和11630个差异表达基因(Deg)。冻害后，天优2号的GO主要参与“大分子生物合成过程”，龙优6号的GO主要参与“刺激反应”和“氧化还原酶活性”。形态和生理指标表明，龙优6号保持了比提纳优2号更高的基础抗寒性，冷驯化可以增强其基础抗寒性。冷冻胁迫可以激活MAPK信号级联，龙油6号的磷酸化水平比天油2号高。根据我们的研究结果，推测甘蓝型油菜细胞膜感知外界信号，然后通过冷激活的MAPK级联和钙相关蛋白激酶途径传递到细胞核，从而导致下游靶基因的激活，从而增强油菜的抗寒性。
Winter rapeseed (Brassica rapa L.) is an important oilseed crop in northwest China. Freezing stress severely limits its production and geographical distribution, and frequent extreme freezing events caused by climate change are increasing the chances of winter freeze-injury. However, the underlying mechanism of B. rapa response to freezing stress remains elusive. Here, B. rapa genome (v3.0) was used as a reference for the comparative transcriptomic analysis of Longyou 6 and Tianyou 2 (strong and weak cold tolerance, respectively) under different freezing stress. Before and after freezing stress, 5,982 and 11,630 unique differentially expressed genes (DEGs) between two cultivars were identified, respectively. After freezing stress, the GO terms in Tianyou 2 were mainly involved in “macromolecule biosynthetic process”, and those in Longyou 6 were involved in “response to stimulus” and “oxidoreductase activity”. Morphological and physiological results indicated that Longyou 6 retained a higher basal freezing resistance than Tinayou 2, and that cold acclimation could strengthen the basal freezing resistance. Freezing stress could activate the MAPK signal cascades, and the phosphorylation level of Longyou 6 showed a higher increase in response to freezing treatment than Tianyou 2. Based on our findings, it was speculated that the cell membrane of B. rapa perceives external signals under freezing stress, which are then transmitted to the nucleus through the cold-activated MAPK cascades and Ca2+-related protein kinase pathway, thus leading to activation of downstream target genes to enhance the freezing resistance of B. rapa.